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Structure Characterization and Immunomodulating Effects of Polysaccharides Isolated from Dendrobium officinale
Wei W.1; Feng L.1,2; Bao W.-R.1; Ma D.-L.3; Leung C.-H.4; Nie S.-P.2; Han Q.-B.1
2016
Source PublicationJournal of Agricultural and Food Chemistry
ISSN0021-8561
Volume64Issue:4Pages:881-889
Abstract

A crude polysaccharide fraction (cDOP) has been determined to be the characteristic marker of Dendrobium officinale, an expensive tea material in Asia, but its chemistry and bioactivity have not been studied. In work reported here, cDOP was destarched (DOP, 90% yield) and separated into two subfraction polysaccharides, DOPa and DOPb, which were characterized by monosaccharide composition and methylation analyses and spectral analyses (FT-IR and 1H and 13C NMR). Both are composed of mannose and glucose at similar ratios and have a similar structure with a backbone of 1,4-linked β-d-mannopyranosyl and β-d-glucopyranosyl residues. Significant differences were observed only in their molecular weights. Bioassay using mouse macrophage cell line RAW264.7 indicated that DOP and its two subfractions enhance cell proliferation, TNF-α secretion, and phagocytosis in a dose-dependent manner. They also induced the proliferation of lymphocytes alone and with mitogens. DOPa and DOPb are thus proven to be major, active polysaccharide markers of D. officinale. 

KeywordChemical Structure Dendrobium Officinale Lymphocytes Polysaccharides Raw264.7 Cells
DOI10.1021/acs.jafc.5b05180
URLView the original
Indexed BySCIE
Language英语
WOS Research AreaAgriculture ; Chemistry ; Food Science & Technology
WOS SubjectAgriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology
WOS IDWOS:000369557200018
The Source to ArticleScopus
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Cited Times [WOS]:58   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorNie S.-P.; Han Q.-B.
Affiliation1.‎ Hong Kong Baptist Univ, Sch Chinese Med, Hong Kong, Hong Kong, Peoples R China
2.Nanchang Univ, State Key Lab Food Sci & Technol, Nanchang, Jiangxi, Peoples R China
3.Nanchang Univ, State Key Lab Food Sci & Technol, Nanchang, Jiangxi, Peoples R China
4.Hong Kong Baptist Univ, Dept Chem, Hong Kong, Hong Kong, Peoples R China
Recommended Citation
GB/T 7714
Wei W.,Feng L.,Bao W.-R.,et al. Structure Characterization and Immunomodulating Effects of Polysaccharides Isolated from Dendrobium officinale[J]. Journal of Agricultural and Food Chemistry,2016,64(4):881-889.
APA Wei W..,Feng L..,Bao W.-R..,Ma D.-L..,Leung C.-H..,...&Han Q.-B..(2016).Structure Characterization and Immunomodulating Effects of Polysaccharides Isolated from Dendrobium officinale.Journal of Agricultural and Food Chemistry,64(4),881-889.
MLA Wei W.,et al."Structure Characterization and Immunomodulating Effects of Polysaccharides Isolated from Dendrobium officinale".Journal of Agricultural and Food Chemistry 64.4(2016):881-889.
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