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Chemical investigation of saponins in different parts of Panax notoginseng by pressurized liquid extraction and liquid chromatography-electrospray ionization-tandem mass spectrometry
Wan J.-B.1,2; Zhang Q.-W.1,2; Hong S.-J.2; Li P.1,2; Li S.-P.1,2; Wang Y.-T.1,2
2012
Source PublicationMolecules
ISSN14203049
Volume17Issue:5Pages:5836
Abstract

A pressurized liquid extraction (PLE) and high performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS) method was developed for the qualitative determination of saponins in different parts of P. notoginseng, including rhizome, root, fibre root, seed, stem, leaf and flower. The samples were extracted using PLE. The analysis was achieved on a Zorbax SB-C18 column with gradient elution of acetonitrile and 8 mM aqueous ammonium acetate as mobile phase. The mass spectrometer was operated in the negative ion mode using the electrospray ionization, and a collision induced dissociation (CID) experiment was also carried out to aid the identification of compounds. Forty one saponins were identified in different parts of P. notoginseng according to the fragmentation patterns and literature reports, among them, 21 saponins were confirmed by comparing the retention time and ESI-MS data with those of standard compounds. The results showed that the chemical characteristics were obviously diverse in different parts of P. notoginseng, which is helpful for pharmacological evaluation and quality control of P. notoginseng. © 2012 by the authors.

KeywordDifferent Parts Of Panax Notoginseng Liquid Chromatography-mass Spectrometry Pressurized Liquid Extraction Saponin
DOIhttp://doi.org/10.3390/molecules17055836
URLView the original
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS IDWOS:000304587600082
The Source to ArticleScopus
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Cited Times [WOS]:32   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.State Key Laboratory of Quality Research in Chinese Medicine (University of Macau), Macao, China
2.Institute of Chinese Medical Sciences, University of Macau, Macao, China
First Author AffilicationUniversity of Macau;  Institute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Wan J.-B.,Zhang Q.-W.,Hong S.-J.,et al. Chemical investigation of saponins in different parts of Panax notoginseng by pressurized liquid extraction and liquid chromatography-electrospray ionization-tandem mass spectrometry[J]. Molecules,2012,17(5):5836.
APA Wan J.-B.,Zhang Q.-W.,Hong S.-J.,Li P.,Li S.-P.,&Wang Y.-T..(2012).Chemical investigation of saponins in different parts of Panax notoginseng by pressurized liquid extraction and liquid chromatography-electrospray ionization-tandem mass spectrometry.Molecules,17(5),5836.
MLA Wan J.-B.,et al."Chemical investigation of saponins in different parts of Panax notoginseng by pressurized liquid extraction and liquid chromatography-electrospray ionization-tandem mass spectrometry".Molecules 17.5(2012):5836.
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