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Bisdemethoxycurcumin suppresses MCF-7 cells proliferation by inducing ROS accumulation and modulating senescence-related pathways
Li Y.-B.1,2; Gao J.-L.3,4; Zhong Z.-F.1; Hoi P.-M.1; Lee S.M.-Y.1; Wang Y.-T.1
2013
Source PublicationPharmacological Reports
ISSN17341140
Volume65Issue:3Pages:700
Abstract

Background: Bisdemethoxycurcumin (BDMC) is a natural derivative of curcumin present in the phenolic components extracted from the dried rhizome of Curcuma longa L. BDMC demonstrated potential chemotherapeutic activities but the underlying mechanisms have not been fully clarified. In the present study, the role of reactive oxidative species (ROS) in the anti-cancer effects of BDMC was investigated. Methods: MCF-7 cells were exposed to BDMC, and then the cell proliferation, colony formation ability and cell cycle profile were analyzed. Cellular ROS level was determined by flow cytometry and fluorescent microscope observation using specific fluorescent probes. Mitochondrial membrane potential (ψm) was assessed using JC-1. In addition, effects of BDMC on senescence-related molecules were analyzed by western blot assay. Results: BDMC significantly inhibited MCF-7 breast cancer cell proliferation, while a rapid rise of the intracellular ROS level accompanied with a reduction of Δψm were observed. In addition, BDMC activated the pro-apoptotic protein p53 and its downstream effector p21 as well as the cell cycle regulatory proteins p16 and its downstream effector retinoblastoma protein (Rb). All of these BDMC-induced effects were counteracted with the pre-incubation of the antioxidant N-acetylcysteine (NAC). Conclusions: These results suggested that BDMC-induced ROS accumulation may contribute to its inhibitory effect on MCF-7 cell viability through regulation of p53/p21 and p16/Rb pathways. Copyright © 2013 by Institute of Pharmacology Polish Academy of Sciences.

KeywordBisdemethoxycurcumin (Bdmc) Breast Cancer Reactive Oxidative Species (Ros) Rhizoma Curcumae Longae
DOIhttp://doi.org/10.1016/S1734-1140(13)71048-X
URLView the original
Indexed BySCI
Language英语
WOS Research AreaPharmacology & Pharmacy
WOS SubjectPharmacology & Pharmacy
WOS IDWOS:000330270400018
The Source to ArticleScopus
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Cited Times [WOS]:28   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorLi Y.-B.; Wang Y.-T.
Affiliation1.Institute of Chinese Medical Sciences, State Key Laboratory of Quality Research in Chinese Medicine, University of Macau, Av. Padre Tomás Pereira S.J., Taipa, Macao 999078, China
2.State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Xueyuan Rd., Haidian District, 100191, Beijing, China
3.Molecular Oncology Laboratory, University of Chicago Medical Center, 5841 South Maryland Av., MC 3079, Chicago, IL 60637, USA
4.Institute of Materia Medica, Zhejiang Chinese Medical University, 548 Binwen Rd., Binjiang District, 310053, Zhejiang, China
First Author AffilicationInstitute of Chinese Medical Sciences
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Li Y.-B.,Gao J.-L.,Zhong Z.-F.,et al. Bisdemethoxycurcumin suppresses MCF-7 cells proliferation by inducing ROS accumulation and modulating senescence-related pathways[J]. Pharmacological Reports,2013,65(3):700.
APA Li Y.-B.,Gao J.-L.,Zhong Z.-F.,Hoi P.-M.,Lee S.M.-Y.,&Wang Y.-T..(2013).Bisdemethoxycurcumin suppresses MCF-7 cells proliferation by inducing ROS accumulation and modulating senescence-related pathways.Pharmacological Reports,65(3),700.
MLA Li Y.-B.,et al."Bisdemethoxycurcumin suppresses MCF-7 cells proliferation by inducing ROS accumulation and modulating senescence-related pathways".Pharmacological Reports 65.3(2013):700.
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