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Danshensu is the major marker for the antioxidant and vasorelaxation effects of Danshen (Salvia miltiorrhiza) water-extracts produced by different heat water-extractions
Zhou X.1; Chan S.W.2; Tseng H.L.3; Deng Y.1; Hoi P.M.3; Choi P.S.3; Or P.M.Y.1; Yang J.-M.4; Lam F.F.Y.1; Lee S.M.Y.3; Leung G.P.H.5; Kong S.K.6; Ho H.P.7; Kwan Y.W.1; Yeung J.H.K.1
2012
Source PublicationPhytomedicine
ISSN9447113
Volume19Issue:14Pages:1263
Abstract

Some of the major components of Danshen (Salvia miltiorrhiza), a widely used Chinese herbal medicine rich in phenolic acids, are thermosensitive and may degrade to other phenolic acids during extractions with heating. The chemical profiles of Danshen water-extract may vary with different heat water extraction at different temperatures, affecting the composition and bioactivity of the extracts. In this study, six water-extracts of Danshen obtained from heat reflux water extraction and microwave-assisted extraction with water (MAE-W) at different temperatures were tested for their composition and pharmacological effects. Among these extracts, the third-round MAE-W (100 °C) extract had the highest phenolic acids and tanshinones contents, with the strongest antioxidant activity in 2,2-diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl (DPPH) assay and ferric reducing/antioxidant potential (FRAP) assay. This extract also showed the strongest inhibitory effects on 2,2′-azobis-2-amidinopropane (AAPH)-induced hemolysis in human red blood cells, hydrogen peroxide-induced apoptosis in rat heart H9c2 cells and the highest relaxation effects on rat basilar artery. The antioxidant effects of Danshen water-extracts linearly correlated to their relaxation effects (r = 0.895-0.977). Through multiple linear regression analysis, danshensu was found to be the most significant marker in the antioxidant and vasodilation effects of Danshen water-extract, while tanshinone IIA as the marker on hydrogen peroxide-induced apoptosis in rat heart H9c2 cells. Danshensu is, therefore, a useful marker for the quality control of Danshen water-extracts in antioxidant and vasodilation, while tanshinone IIA for anti-apoptotic potential of different extracts. © 2012 Elsevier GmbH.

KeywordAntioxidant Microwave Water Extraction Phenolic Acid Salvia Miltiorrhiza Tanshinones Vascular Relaxation
DOI10.1016/j.phymed.2012.08.011
URLView the original
Indexed BySCI
Language英语
WOS Research AreaPlant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
WOS SubjectPlant Sciences ; Chemistry, Medicinal ; Integrative & Complementary Medicine ; Pharmacology & Pharmacy
WOS IDWOS:000313774000006
The Source to ArticleScopus
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Cited Times [WOS]:43   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China
2.State Key Laboratory of Chinese Medicine and Molecular Pharmacology, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hong Kong, China
3.Institute of Chinese Medical Sciences, University of Macau, Macao, China
4.School of Chinese Medicine, The Chinese University of Hong Kong, Hong Kong, China
5.Department of Pharmcology and Pharmacy, The University of Hong Kong, Hong Kong, China
6.School of Life Sciences, Faculty of Sciences, The Chinese University of Hong Kong, Hong Kong, China
7.Department of Electronic Engineering, Faculty of Engineering, The Chinese University of Hong Kong, Hong Kong, China
Recommended Citation
GB/T 7714
Zhou X.,Chan S.W.,Tseng H.L.,et al. Danshensu is the major marker for the antioxidant and vasorelaxation effects of Danshen (Salvia miltiorrhiza) water-extracts produced by different heat water-extractions[J]. Phytomedicine,2012,19(14):1263.
APA Zhou X..,Chan S.W..,Tseng H.L..,Deng Y..,Hoi P.M..,...&Yeung J.H.K..(2012).Danshensu is the major marker for the antioxidant and vasorelaxation effects of Danshen (Salvia miltiorrhiza) water-extracts produced by different heat water-extractions.Phytomedicine,19(14),1263.
MLA Zhou X.,et al."Danshensu is the major marker for the antioxidant and vasorelaxation effects of Danshen (Salvia miltiorrhiza) water-extracts produced by different heat water-extractions".Phytomedicine 19.14(2012):1263.
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