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Characterization and discrimination of polysaccharides from different species of Cordyceps using saccharide mapping based on PACE and HPTLC
Wu D.-T.; Cheong K.-L.; Wang L.-Y.; Lv G.-P.; Ju Y.-J.; Feng K.; Zhao J.; Li S.-P.
2014
Source PublicationCarbohydrate Polymers
ISSN1448617
Volume103Issue:1Pages:100
Abstract

Polysaccharides from seven species of natural and cultured Cordyceps were firstly investigated and compared using saccharide mapping, partially acidic/enzymatic (α-amylase, β-glucanase and pectinase) digestion followed with polysaccharide analysis by using carbohydrate gel electrophoresis (PACE) and high performance thin layer chromatography (HPTLC) analysis, respectively, to obtain the comprehensive profiles of hydrolysates of the polysaccharides and their characters. The results showed that 1,4-α-d-glucosidic, 1,4-β-d-glucosidic and 1,4-α-d-galactosidic linkages were existed in natural and cultured Cordyceps sinensis, cultured Cordyceps militaris, natural Cordyceps gracilis and Cordyceps ciecadae. The similarity of polysaccharides from cultured C. militaris to natural C. sinensis was relatively high, which might contribute to the rational use of C. militaris. Moreover, different species of natural and cultured Cordyceps can be differentiated based on the saccharide mapping, which is helpful to well understand the structural characters of polysaccharides from different species of Cordyceps and to improve the quality control of polysaccharides in natural and cultured Cordyceps. © 2013 Elsevier Ltd.

KeywordCordyceps Militaris Cordyceps Sinensis Hptlc Pace Polysaccharide Saccharide Mapping
DOIhttp://doi.org/10.1016/j.carbpol.2013.12.034
URLView the original
Indexed BySCI
Language英语
WOS Research AreaChemistry ; Polymer Science
WOS SubjectChemistry, Applied ; Chemistry, Organic ; Polymer Science
WOS IDWOS:000332812600013
The Source to ArticleScopus
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Cited Times [WOS]:32   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorZhao J.; Li S.-P.
AffiliationState Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao
First Author AffilicationInstitute of Chinese Medical Sciences
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Wu D.-T.,Cheong K.-L.,Wang L.-Y.,et al. Characterization and discrimination of polysaccharides from different species of Cordyceps using saccharide mapping based on PACE and HPTLC[J]. Carbohydrate Polymers,2014,103(1):100.
APA Wu D.-T..,Cheong K.-L..,Wang L.-Y..,Lv G.-P..,Ju Y.-J..,...&Li S.-P..(2014).Characterization and discrimination of polysaccharides from different species of Cordyceps using saccharide mapping based on PACE and HPTLC.Carbohydrate Polymers,103(1),100.
MLA Wu D.-T.,et al."Characterization and discrimination of polysaccharides from different species of Cordyceps using saccharide mapping based on PACE and HPTLC".Carbohydrate Polymers 103.1(2014):100.
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