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Development of an Iridium(III) Complex as a G-Quadruplex Probe and Its Application for the G-Quadruplex-Based Luminescent Detection of Picomolar Insulin
Wang M.; Wang W.; Kang T.-S.; Leung C.-H.; Ma D.-L.
2016
Source PublicationAnalytical Chemistry
ISSN32700
Volume88Issue:1Pages:981
AbstractIn this study, an unreported Ir(III) complex 1 was identified by screening as a versatile G-quadruplex probe. It exhibited highly selective response for different G-quadruplex DNA over double strand, single strand and triplex DNA. Compared with the organic G-quadruplex probe thioflavin T, complex 1 displays a longer lifetime, a larger Stokes shift, comparable G-quadruplex/ssDNA enhancement ratios, and higher G-quadruplex/triplex DNA enhancement ratios. In consideration of the encouraging G-quadruplex probe performance of complex 1, we employed 1 to develop a G-quadruplex-based detection system for the detection of insulin as a "proof-of-principle" concept. We also demonstrate an optimization process that enhanced the sensitivity of this sensing assay. Compared to previously reported methods, our "mix-and-detect" detection methodology is easy operated, quick, and cost-effective. A detection limit as low as 80 pM for insulin can be achieved by this sensing approach, with a linear relationship between luminescence intensity and insulin concentration established from 80 pM to 20 nM. Moreover, this assay could work effectively in diluted human serum. © 2015 American Chemical Society.
DOI10.1021/acs.analchem.5b04064
URLView the original
Language英语
The Source to ArticleScopus
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被引频次[WOS]:49   [WOS记录]     [WOS相关记录]
Document TypeJournal article
专题University of Macau
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Wang M.,Wang W.,Kang T.-S.,et al. Development of an Iridium(III) Complex as a G-Quadruplex Probe and Its Application for the G-Quadruplex-Based Luminescent Detection of Picomolar Insulin[J]. Analytical Chemistry,2016,88(1):981.
APA Wang M.,Wang W.,Kang T.-S.,Leung C.-H.,&Ma D.-L..(2016).Development of an Iridium(III) Complex as a G-Quadruplex Probe and Its Application for the G-Quadruplex-Based Luminescent Detection of Picomolar Insulin.Analytical Chemistry,88(1),981.
MLA Wang M.,et al."Development of an Iridium(III) Complex as a G-Quadruplex Probe and Its Application for the G-Quadruplex-Based Luminescent Detection of Picomolar Insulin".Analytical Chemistry 88.1(2016):981.
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