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Indirubin shows anti-angiogenic activity in an in vivo zebrafish model and an in vitro HUVEC model
Alex D.1; Lam I.K.1; Lin Z.2; Lee S.M.Y.1
2010
Source PublicationJournal of Ethnopharmacology
ISSN3788741
Volume131Issue:2Pages:242
Abstract

Aim of the study: Indirubin is an active ingredient of the traditional Chinese medicine, Dang Gui Long Hui Wan, commonly used for the treatment of chronic myelocytic leukemia (CML) and other inflammatory conditions. These anti-leukemic and anti-inflammatory activities may be mediated by anti-angiogenic action. To investigate the anti-angiogenic activity of indirubin, we tested its inhibitory effect on blood vessel formation in zebrafish embryos and on endothelial cell proliferation in culture. Materials and methods: The anti-angiogenic activity of indirubin was tested using transgenic zebrafish embryos with fluorescent vasculature and human umbilical vein endothelial cells (HUVECs). Apoptosis was analyzed with a terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) assay. Results: Indirubin dose-dependently inhibited intersegmental vessel formation in zebrafish embryos. It also inhibited HUVEC proliferation by the induction of cellular apoptosis and cell-cycle arrest at the G0/G1 phase. Conclusions: The anti-angiogenic activity of indirubin may partly contribute to its anti-leukemic and anti-psoriatic properties and may be valuable for the treatment of diseases with excessive angiogenesis. The zebrafish model of angiogenesis was further validated in this study. © 2010.

KeywordAnti-angiogenesis Apoptosis Indirubin Zebrafish
DOI10.1016/j.jep.2010.05.016
URLView the original
Indexed BySCI
Language英语
WOS Research AreaPlant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
WOS SubjectPlant Sciences ; Chemistry, Medicinal ; Integrative & Complementary Medicine ; Pharmacology & Pharmacy
WOS IDWOS:000282709100002
The Source to ArticleScopus
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Cited Times [WOS]:30   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorLee S.M.Y.
Affiliation1.Institute of Chinese Medical Sciences, University of Macau, Av. Padre Tomás Pereira S.J., Taipa, Macao, China
2.School of Chinese Medicine, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong SAR, China
First Author AffilicationInstitute of Chinese Medical Sciences
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Alex D.,Lam I.K.,Lin Z.,et al. Indirubin shows anti-angiogenic activity in an in vivo zebrafish model and an in vitro HUVEC model[J]. Journal of Ethnopharmacology,2010,131(2):242.
APA Alex D.,Lam I.K.,Lin Z.,&Lee S.M.Y..(2010).Indirubin shows anti-angiogenic activity in an in vivo zebrafish model and an in vitro HUVEC model.Journal of Ethnopharmacology,131(2),242.
MLA Alex D.,et al."Indirubin shows anti-angiogenic activity in an in vivo zebrafish model and an in vitro HUVEC model".Journal of Ethnopharmacology 131.2(2010):242.
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