UM  > 中華醫藥研究院
Stereoselective interactions of lactic acid enantiomers with HSA: Spectroscopy and docking application
Mu, Hongtao1; Chen, Shaohuan1; Liu, Fengyin1; Xiao, Jianbo2; Huang, Hui3; Zhang, Yuhua1; Sun, Yuanming4; Gao, Xiangyang5; Lei, Hongtao4; Yuan, Xuewen1
2019-01
Source PublicationFOOD CHEMISTRY
ISSN0308-8146
Volume270Pages:429-435
Abstract

Lactic acid enantiomers, normally found in fermented food, are absorbed into the blood and interact with plasma carrier protein human serum albumin (HSA). Unveiling the effect on the function and structure of HSA during chiral interaction can give a better understanding of the different distribution activities of the two enantiomers. Multi-spectroscopic methods and molecular modelling techniques are used to study the interactions between lactic acid enantiomers and HSA. Time-resolved and steady-state fluorescence spectra manifest that the fluorescence quenching mechanism is mainly static in type, due to complex formation. Binding interactions, deduced by thermodynamic calculation, agree with the docking prediction. Docking results and kinetic constants represent chiral-recognizing discriminations consistently. The bindings of lactic acid enantiomers lead to some microenvironmental and slight conformational changes of HSA as shown by circular dichroism (CD), synchronous and three-dimensional fluorescence spectra. This investigation may yield useful information about the possible toxicity risk of lactic acid enantiomers to human health.

KeywordLactic Acid Isomers Human Serum Albumin Fluorescence Spectroscopy Circular Dichroism Docking Simulation
DOI10.1016/j.foodchem.2018.07.135
URLView the original
Indexed BySCI
Language英语
WOS Research AreaChemistry ; Food Science & Technology ; Nutrition & Dietetics
WOS SubjectChemistry, Applied ; Food Science & Technology ; Nutrition & Dietetics
WOS IDWOS:000443253200056
PublisherELSEVIER SCI LTD
The Source to ArticleWOS
Fulltext Access
Citation statistics
Cited Times [WOS]:10   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorLei, Hongtao; Yuan, Xuewen
Affiliation1.College of Biology and Food Engineering, Guangdong University of Education, Guangzhou 510303, China
2.Institute of Chinese Medical Sciences, State Key Laboratory of Quality Research in Chinese Medicine, University of Macau Taipa, Macau
3.South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China
4.Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangdong Provincial Engineering & Technique Research Centre of Food Safety Detection and Risk Assessment, Guangzhou 510642, China
5.College of Food Science, South China Agricultural University, Guangzhou 510642, China
Recommended Citation
GB/T 7714
Mu, Hongtao,Chen, Shaohuan,Liu, Fengyin,et al. Stereoselective interactions of lactic acid enantiomers with HSA: Spectroscopy and docking application[J]. FOOD CHEMISTRY,2019,270:429-435.
APA Mu, Hongtao.,Chen, Shaohuan.,Liu, Fengyin.,Xiao, Jianbo.,Huang, Hui.,...&Yuan, Xuewen.(2019).Stereoselective interactions of lactic acid enantiomers with HSA: Spectroscopy and docking application.FOOD CHEMISTRY,270,429-435.
MLA Mu, Hongtao,et al."Stereoselective interactions of lactic acid enantiomers with HSA: Spectroscopy and docking application".FOOD CHEMISTRY 270(2019):429-435.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Mu, Hongtao]'s Articles
[Chen, Shaohuan]'s Articles
[Liu, Fengyin]'s Articles
Baidu academic
Similar articles in Baidu academic
[Mu, Hongtao]'s Articles
[Chen, Shaohuan]'s Articles
[Liu, Fengyin]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Mu, Hongtao]'s Articles
[Chen, Shaohuan]'s Articles
[Liu, Fengyin]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.