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Anti-angiogenic effect of furanodiene on HUVECs in vitro and on zebrafish in vivo
Zhong Z.-F.1,2,3; Hoi P.-M.1,2; Wu G.-S.1,2; Xu Z.-T.1,2; Tan W.1,2; Chen X.-P.1,2; Cui L.3; Wu T.3; Wang Y.-T.1,2
2012
Source PublicationJournal of Ethnopharmacology
ISSN3788741
Volume141Issue:2Pages:721
Abstract

Ethnopharmacological relevance: Furanodiene is an active ingredient of the traditional Chinese medicine, Rhizoma Curcumae, commonly used for the treatment of cancer in China. Aim of the study: To investigate the anti-cancer property of Rhizoma Curcumae, this study describes the anti-angiogenic activities of furanodiene in human umbilical vein endothelial cells (HUVECs) in vitro and in zebrafish in vivo. Materials and methods: HUVECs were treated with different doses of furanodiene in the presence or absence of vascular endothelial growth factor (VEGF). The anti-proliferative effect of furanodiene was measured using the XTT assay. The anti-migration and anti-invasion activities of this compound were investigated with a wound-healing migration model and a three-dimensional cell invasion model, respectively. The effects of furanodiene on HUVEC differentiation were assessed by in vitro tube formation in Matrigel™. The expression of related proteins was detected by Western blot. Morphological observations of zebrafish were evaluated in transgenic Tg (fli1: EGFP) zebrafish embryos. Results: Our results showed that furanodiene exposure could significantly inhibit the proliferation of HUVECs in a dose-dependent manner and inhibit VEGF-induced proliferation at a low dose. Relative to the VEGF-induced control, the number of invading and migrating cells was significantly reduced in the furanodiene-treated groups. Furanodiene also dramatically suppressed tube formation and p-Akt (Ser473), p-Erk 1/2 (Thr202/Tyr204), ICAM-1, p-p85 (Ser428) as well as p85 protein expression. Furthermore, exposure to furanodiene inhibited angiogenesis in the zebrafish model. Conclusions: This study demonstrated that furanodiene exposure exhibits a potential anti-angiogenic effect through suppression of endothelial cell growth, invasion, migration and tube formation via regulation of the PI3K pathway. This potential anti-angiogenic effect of furanodiene may play an important role in the anti-tumor activity of the traditional Chinese medicine, Rhizoma Curcumae. © 2011 Elsevier Ireland Ltd. All rights reserved.

KeywordAnti-angiogenesis Furanodiene Invasion Migration Tube Formation Zebrafish
DOIhttp://doi.org/10.1016/j.jep.2011.08.052
URLView the original
Indexed BySCI
Language英语
WOS Research AreaPlant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
WOS SubjectPlant Sciences ; Chemistry, Medicinal ; Integrative & Complementary Medicine ; Pharmacology & Pharmacy
WOS IDWOS:000305201100026
The Source to ArticleScopus
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Cited Times [WOS]:36   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorWang Y.-T.
Affiliation1.State Key Laboratory of Quality Research in Chinese Medicine, Macau, China
2.Institute of Chinese Medical Sciences, University of Macau, Macau, China
3.Department of Pharmacology, Guangdong Medical College, Zhanjiang, China
First Author AffilicationInstitute of Chinese Medical Sciences
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Zhong Z.-F.,Hoi P.-M.,Wu G.-S.,et al. Anti-angiogenic effect of furanodiene on HUVECs in vitro and on zebrafish in vivo[J]. Journal of Ethnopharmacology,2012,141(2):721.
APA Zhong Z.-F..,Hoi P.-M..,Wu G.-S..,Xu Z.-T..,Tan W..,...&Wang Y.-T..(2012).Anti-angiogenic effect of furanodiene on HUVECs in vitro and on zebrafish in vivo.Journal of Ethnopharmacology,141(2),721.
MLA Zhong Z.-F.,et al."Anti-angiogenic effect of furanodiene on HUVECs in vitro and on zebrafish in vivo".Journal of Ethnopharmacology 141.2(2012):721.
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