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Synergistic anti-cancer activity of the combination of dihydroartemisinin and doxorubicin in breast cancer cells
Wu G.-S.1; Lu J.-J.1,2; Guo J.-J.1; Huang M.-Q.3; Gan L.2; Chen X.-P.1; Wang Y.-T.1
2013
Source PublicationPharmacological Reports
ISSN17341140
Volume65Issue:2Pages:453
Abstract

Background

Dihydroartemisinin (DHA) exhibits potent anti-malarial and anti-cancer activities. This study aimed to investigate the anti-proliferative effects of a combination of DHA and doxorubicin(DOX) on human breast cancer cells.

Methods

MTT assay and the combination index (CI) were used to show the anti-proliferative effects and calculate the synergism potential, respectively. Flow cytometry assay was used to detect apoptosis and the intracellular accumulation of DOX. JC-1 staining was used to determine the mitochondrial membrane potentialWestern blot analysis was used to detect the protein expression of some apoptosis-related molecules.

Results

Asynergistic anti-proliferative effect was found, and the enhanced anti-cancer activity was observed to be accompanied by the prompt onset of apoptosis in MCF-7 cells. The combinative treatment remarkably decreased the mitochondrial membrane potential and activated caspase cascades more than the mono-treatment. Pretreatment with DHAalso did not influence the accumulation of DOX in MCF-7 cells.

Conclusion

This study presented a new opportunity to enhance the effectiveness of future treatment regimens of breast cancer using DOX.

KeywordAnti-tumor Apoptosis Dihydroartemisinin Doxorubicin Mcf-7 Synergistic
DOIhttp://doi.org/10.1016/S1734-1140(13)71021-1
URLView the original
Indexed BySCI
Language英语
WOS Research AreaPharmacology & Pharmacy
WOS SubjectPharmacology & Pharmacy
WOS IDWOS:000330270300020
The Source to ArticleScopus
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Cited Times [WOS]:37   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorWang Y.-T.
Affiliation1.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China
2.College of Life Sciences, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, 310053, China
3.College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian, 350108, China
First Author AffilicationInstitute of Chinese Medical Sciences
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Wu G.-S.,Lu J.-J.,Guo J.-J.,et al. Synergistic anti-cancer activity of the combination of dihydroartemisinin and doxorubicin in breast cancer cells[J]. Pharmacological Reports,2013,65(2):453.
APA Wu G.-S..,Lu J.-J..,Guo J.-J..,Huang M.-Q..,Gan L..,...&Wang Y.-T..(2013).Synergistic anti-cancer activity of the combination of dihydroartemisinin and doxorubicin in breast cancer cells.Pharmacological Reports,65(2),453.
MLA Wu G.-S.,et al."Synergistic anti-cancer activity of the combination of dihydroartemisinin and doxorubicin in breast cancer cells".Pharmacological Reports 65.2(2013):453.
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