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Platycodin D induces apoptosis, and inhibits adhesion, migration and invasion in HepG2 hepatocellular carcinoma cells
Li T.1; Xu W.-S.1; Wu G.-S.1; Chen X.-P.1; Wang Y.-T.1; Lu J.-J.1,2
2014
Source PublicationAsian Pacific Journal of Cancer Prevention
ISSN15137368
Volume15Issue:4Pages:1745
Abstract

Background: Platycodin D (PD), a triterpenoid saponin isolated from the Chinese medicinal herb Platycodonis radix, possesses anti-cancer effects in several cancer cell lines. The aim of this study was to evaluate its anti-cancer activities in hepatocellular carcinoma cells. Materials and Methods: MTT and colony formation assays were performed to evaluate cell proliferation, along with flow cytometry and Western blotting for apoptosis. Cell adhesion was tested by observing cellular morphology under a microscope, while the transwell assay was employed to investigate the cell migration and invasion. Results: PD concentration-dependently inhibited cell proliferation in both HepG2 and Hep3B cells, and significantly suppressed colony formation and induced apoptosis in HepG2 cells. The protein levels of cleaved poly ADP-ribose polymerase (PARP) and Bax were up-regulated while that of survivin was down-regulated after treatment with PD. Moreover, PD not only obviously suppressed the adhesion of HepG2 cells to Matrigel, but also remarkably depressed their migration and invasion induced by 12-O-tetradecanoylphorbol 13-acetate (TPA) . Conclusions: PD presents anti-cancer potential in hepatocellular carcinoma cells via inducing apoptosis, and inhibiting cell adhesion, migration and invasion, indicating promising features as a lead compound for anti-cancer agent development.

KeywordApoptosis Cancer Hepg2 Metastasis Platycodin d
DOIhttp://doi.org/10.7314/APJCP.2014.15.4.1745
URLView the original
Indexed BySCI
Language英语
WOS Research AreaOncology
WOS SubjectOncology
WOS IDWOS:000333670100045
The Source to ArticleScopus
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Cited Times [WOS]:36   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorWang Y.-T.; Lu J.-J.
Affiliation1.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao
2.College of life Sciences, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China
First Author AffilicationInstitute of Chinese Medical Sciences
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Li T.,Xu W.-S.,Wu G.-S.,et al. Platycodin D induces apoptosis, and inhibits adhesion, migration and invasion in HepG2 hepatocellular carcinoma cells[J]. Asian Pacific Journal of Cancer Prevention,2014,15(4):1745.
APA Li T.,Xu W.-S.,Wu G.-S.,Chen X.-P.,Wang Y.-T.,&Lu J.-J..(2014).Platycodin D induces apoptosis, and inhibits adhesion, migration and invasion in HepG2 hepatocellular carcinoma cells.Asian Pacific Journal of Cancer Prevention,15(4),1745.
MLA Li T.,et al."Platycodin D induces apoptosis, and inhibits adhesion, migration and invasion in HepG2 hepatocellular carcinoma cells".Asian Pacific Journal of Cancer Prevention 15.4(2014):1745.
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