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Determination of cell metabolite VEGF165 and dynamic analysis of protein–DNA interactions by combination of microfluidic technique and luminescent switch-on probe
Lin X.1,2,3; Leung K.-H.3; Lin L.1; Lin L.1; Lin S.3; Leung C.-H.4; Ma D.-L.3; Lin J.-M.1
2016
Source PublicationBiosensors and Bioelectronics
ISSN9565663
Volume79Pages:41-47
Abstract

In this paper, we rationally design a novel G-quadruplex-selective luminescent iridium (III) complex for rapid detection of oligonucleotide and VEGF165 in microfluidics. This new probe is applied as a convenient biosensor for label-free quantitative analysis of VEGF165 protein from cell metabolism, as well as for studying the kinetics of the aptamer-protein interaction combination with a microfluidic platform. As a result, we have successfully established a quantitative analysis of VEGF165 from cell metabolism. Furthermore, based on the principles of hydrodynamic focusing and diffusive mixing, different transient states during kinetics process were monitored and recorded. Thus, the combination of microfluidic technique and G-quadruplex luminescent probe will be potentially applied in the studies of intramolecular interactions and molecule recognition in the future.

KeywordFunctional Nucleic Acid Iridium (Iii) Probe Kinetic Analysis Microfluidic Device Vegf165
DOI10.1016/j.bios.2015.11.089
URLView the original
Indexed BySCIE
Language英语
WOS Research AreaBiophysics ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics ; Biotechnology & Applied Microbiology
WOS SubjectBiophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology
WOS IDWOS:000370309200007
The Source to ArticleScopus
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Cited Times [WOS]:46   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorMa D.-L.; Lin J.-M.
Affiliation1.Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, The Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Tsinghua University, Beijing 100084, China
2.College of Chemical Engineering, Huaqiao University, Xiamen 361000, China
3.Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China
4.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China
Recommended Citation
GB/T 7714
Lin X.,Leung K.-H.,Lin L.,et al. Determination of cell metabolite VEGF165 and dynamic analysis of protein–DNA interactions by combination of microfluidic technique and luminescent switch-on probe[J]. Biosensors and Bioelectronics,2016,79:41-47.
APA Lin X..,Leung K.-H..,Lin L..,Lin L..,Lin S..,...&Lin J.-M..(2016).Determination of cell metabolite VEGF165 and dynamic analysis of protein–DNA interactions by combination of microfluidic technique and luminescent switch-on probe.Biosensors and Bioelectronics,79,41-47.
MLA Lin X.,et al."Determination of cell metabolite VEGF165 and dynamic analysis of protein–DNA interactions by combination of microfluidic technique and luminescent switch-on probe".Biosensors and Bioelectronics 79(2016):41-47.
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