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Ultrasensitive electrochemical detection of miRNA-21 by using an iridium(III) complex as catalyst
Miao X.1,2; Wang W.2; Kang T.3; Liu J.2; Shiu K.-K.2; Leung C.-H.3; Ma D.-L.2
Source PublicationBiosensors and Bioelectronics

The ultrasensitive electrochemical detection of miRNA-21 was realized by using a novel redox and catalytic “all-in-one” mechanism with an iridium(III) complex as a catalyst. To construct such a sensor, a capture probe (CP) was firstly immobilized onto the gold electrode surface. In the presence of miRNA-21, a sandwiched DNA complex could form between CP and a methylene blue (MB) labeled G-rich detection probe modified onto a gold nanoparticle (AuNP) surface (DP-AuNPs). Upon addition of K+, the structure of DP changed to a G-quadruplex. Then, the iridium(III) complex could selectively interact with the G-quadruplex, catalyzing the reduction of H2O2, which was accompanied by an electrochemical signal change using MB as an electron mediator. Under optimal conditions, the electrochemical signal of MB reduction peak was proportional to miRNA concentration in the range from 5.0 fM to 1.0 pM, with a detection limit of 1.6 fM. In addition, satisfactory results were obtained for miRNA-21 detection in human serum samples, indicating a potential application of the sensor for bioanalysis. © 2016 Elsevier B.V.

KeywordElectrochemical Biosensor Iridium(Iii) Complex Mirna-21
URLView the original
Indexed BySCI
WOS Research AreaBiophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
WOS SubjectBiophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology
WOS IDWOS:000384853300062
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Cited Times [WOS]:53   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorMa D.-L.
Affiliation1.School of Life Science, Jiangsu Normal University, Xuzhou 221116, PR China
2.Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China
3.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China
Recommended Citation
GB/T 7714
Miao X.,Wang W.,Kang T.,et al. Ultrasensitive electrochemical detection of miRNA-21 by using an iridium(III) complex as catalyst[J]. Biosensors and Bioelectronics,2016,86:454.
APA Miao X..,Wang W..,Kang T..,Liu J..,Shiu K.-K..,...&Ma D.-L..(2016).Ultrasensitive electrochemical detection of miRNA-21 by using an iridium(III) complex as catalyst.Biosensors and Bioelectronics,86,454.
MLA Miao X.,et al."Ultrasensitive electrochemical detection of miRNA-21 by using an iridium(III) complex as catalyst".Biosensors and Bioelectronics 86(2016):454.
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