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Preparation and identification of oligosaccharides in lotus seeds and determination of their distribution in different parts of lotus
Chen, Lingxiao1; Hu, Dejun1; Liang, Xiao2; Zhao, Jing1; Li, Shaoping1
2018-08
Source PublicationELECTROPHORESIS
ISSN0173-0835
Volume39Issue:15Pages:2020-2028
Abstract

Three fractions (I-III) were separated from crude oligosaccharides of lotus seeds by fast protein liquid chromatography with final purity of 97.6, 96.3, and 96.8%, respectively. The fractions were identified as sucrose, raffinose, and stachyose by using TLC, HPLC with charged aerosol detector (CAD), LC-MS, and methylation analysis. Subsequently sucrose and raffinose family oligosaccharides (RFOs) with degree of polymerization (DP) 3-5 (raffinose, stachyose, and verbascose) have been quantified by HPLC-CAD for the first time. All calibration curves for investigated analytes showed good linear regression (R-2>0.9952). Their limit of detection and limit of quantity were in the ranges 0.14-0.28 and 0.36-0.48g/mL, respectively. The recoveries ranged from 96.6 to 103.4%. The contents of sucrose and RFOs DP3-DP5 were different in lotus seeds and other parts of lotus samples, but similar in their own variety. Additionally, the distribution of RFOs in different parts of lotus were also compared and the results indicated that RFOs might be mainly synthesized in lotus seeds. This work is helpful for understanding the way of biosynthesis of RFOs in lotus as well as quality control of plants containing RFOs.

KeywordHplc-cad Lotus Seeds Quantitative Analysis Raffinose Family Oligosaccharides Structural Characterization
DOIhttp://doi.org/10.1002/elps.201700490
URLView the original
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000440549300021
PublisherWILEY
The Source to ArticleWOS
Fulltext Access
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Cited Times [WOS]:1   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorLi, Shaoping
Affiliation1.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao SAR, P. R. China
2.Bino Beijing Limited, Beijing, P. R. China
First Author AffilicationInstitute of Chinese Medical Sciences
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Chen, Lingxiao,Hu, Dejun,Liang, Xiao,et al. Preparation and identification of oligosaccharides in lotus seeds and determination of their distribution in different parts of lotus[J]. ELECTROPHORESIS,2018,39(15):2020-2028.
APA Chen, Lingxiao,Hu, Dejun,Liang, Xiao,Zhao, Jing,&Li, Shaoping.(2018).Preparation and identification of oligosaccharides in lotus seeds and determination of their distribution in different parts of lotus.ELECTROPHORESIS,39(15),2020-2028.
MLA Chen, Lingxiao,et al."Preparation and identification of oligosaccharides in lotus seeds and determination of their distribution in different parts of lotus".ELECTROPHORESIS 39.15(2018):2020-2028.
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