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Quality evaluation of Ganoderma through simultaneous determination of nine triterpenes and sterols using pressurized liquid extraction and high performance liquid chromatography
Zhao J.1; Zhang X.-Q.2; Li S.-P.1; Yang F.-Q.1; Wang Y.-T.1; Ye W.-C.2
2006
Source PublicationJournal of Separation Science
ISSN16159306
Volume29Issue:17Pages:2609
Abstract

A method combining HPLC and pressurized liquid extraction was developed for simultaneous quantification of nine components, including eight triterpenes (ganoderic acid A, ganoderic acid Y, ganoderic acid DM, ganoderol A, ganoderol B, ganoderal A, methyl ganoderate D and ganoderate G) and a sterol (ergosterol), in Ganoderma. The determination was achieved by using a Zorbax ODS C18 analytical column (4.6 × 250 mm id, 5 μm) and gradient elution with diode-array detection. All calibration curves showed good linearity (r2 >0.9997) within the test ranges. The developed method showed good repeatability for the quantification of the nine investigated components in Ganoderma with intra- and inter-day variations of less than 2.4% and 4.1%, respectively. The validated method was successfully applied to quantify the nine components in two species of Ganoderma, i.e. G. lucidum and G. sinense, used as Lingzhi in China. Furthermore, hierarchical clustering analysis based on the nine components in HPLC profiles from the tested 11 samples showed that chemical characteristics were significantly different between G. lucidum and G. sinense, which suggested that clinical investigation should be performed so as to ensure the safety and efficacy of medication. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

KeywordGanoderma Hplc Pressurized Liquid Extraction Sterols Triterpenes
DOIhttp://doi.org/10.1002/jssc.200600178
URLView the original
Indexed BySCI
Language英语
WOS Research AreaChemistry
WOS SubjectChemistry, Analytical
WOS IDWOS:000243338600007
The Source to ArticleScopus
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Cited Times [WOS]:35   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorLi S.-P.
Affiliation1.Institute of Chinese MedicalSciences, University of Macau,Taipa, Macau, P. R. China
2.Institute of TCM & NaturalMedicine, Jinan University,Guangzhou, P. R. China
First Author AffilicationUniversity of Macau
Corresponding Author AffilicationUniversity of Macau
Recommended Citation
GB/T 7714
Zhao J.,Zhang X.-Q.,Li S.-P.,et al. Quality evaluation of Ganoderma through simultaneous determination of nine triterpenes and sterols using pressurized liquid extraction and high performance liquid chromatography[J]. Journal of Separation Science,2006,29(17):2609.
APA Zhao J.,Zhang X.-Q.,Li S.-P.,Yang F.-Q.,Wang Y.-T.,&Ye W.-C..(2006).Quality evaluation of Ganoderma through simultaneous determination of nine triterpenes and sterols using pressurized liquid extraction and high performance liquid chromatography.Journal of Separation Science,29(17),2609.
MLA Zhao J.,et al."Quality evaluation of Ganoderma through simultaneous determination of nine triterpenes and sterols using pressurized liquid extraction and high performance liquid chromatography".Journal of Separation Science 29.17(2006):2609.
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