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Simultaneous determination of five flavonoids in licorice using pressurized liquid extraction and capillary electrochromatography coupled with peak suppression diode array detection
Chen X.J.1; Zhao J.1,2; Meng Q.1; Li S.P.1; Wang Y.T.1
2009
Source PublicationJournal of Chromatography A
ISSN219673
Volume1216Issue:43Pages:7329
Abstract

Pressurized liquid extraction (PLE) and capillary electrochromatography(CEC) methods were developed for the simultaneous determination of five flavonoids, namely liquiritin, isoliquiritin, ononin, liquiriti-genin and isoliquiritigenin, in licorice using baicalein as internal standard (IS). Peak suppression technique was used for the quantification of ononin because of its poor resolution with isoliquiritin. The analysis was performed on a Hypersil C18 capillary (3 μm, 100μm/25cm) with a mixture of10mM phosphate buffer (pH 3.0)/ACN (65:35, v/v) as mobile phase running at 25kV and 30°C. The detection wavelengths were set at 275 nm (without reference wavelength for liquiritin and liquiritigenin), 360 nm (without reference wavelength for isoliquiritin and isoliquiritigenin) and 254nm (withreference wavelength of405nmfor ononin). All calibration curves showed good linearity (R2 >0.9993) within the test ranges. The LOD and LOQ were lower than 2.1 and 8.3 μg/mL, respectively. The RSDs of intra- and interday for relative peak areas of five analytes to IS were less than 3.8 and 4.7%, respectively, and the recoveries were 98.2-103.8%. The validated method was successfully applied to the quantitative analysis of five flavonoids in licorice, which is helpful to its quality control. © 2009 Elsevier B.V.

KeywordCapillary Electrochromatography Flavonoids Licorice Peak Suppression Pressurized Liquid Extraction
DOIhttp://doi.org/10.1016/j.chroma.2009.08.034
URLView the original
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000271108800025
The Source to ArticleScopus
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Cited Times [WOS]:41   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorLi S.P.
Affiliation1.Institute of Chinese Medical Sciences, University of Macau, Taipa, Macao SAR, China
2.Key Laboratory of Modern Chinese Medicines, Ministry of Education and Department of Pharmacognosy, China Pharmaceutical University, Nanjing 210009, China
First Author AffilicationInstitute of Chinese Medical Sciences
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Chen X.J.,Zhao J.,Meng Q.,et al. Simultaneous determination of five flavonoids in licorice using pressurized liquid extraction and capillary electrochromatography coupled with peak suppression diode array detection[J]. Journal of Chromatography A,2009,1216(43):7329.
APA Chen X.J.,Zhao J.,Meng Q.,Li S.P.,&Wang Y.T..(2009).Simultaneous determination of five flavonoids in licorice using pressurized liquid extraction and capillary electrochromatography coupled with peak suppression diode array detection.Journal of Chromatography A,1216(43),7329.
MLA Chen X.J.,et al."Simultaneous determination of five flavonoids in licorice using pressurized liquid extraction and capillary electrochromatography coupled with peak suppression diode array detection".Journal of Chromatography A 1216.43(2009):7329.
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