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Evaluation of carbohydrates in natural and cultured Cordyceps by pressurized liquid extraction and gas chromatography coupled with mass spectrometry
Guan J.; Yang F.-Q.; Li S.-P.
2010
Source PublicationMolecules
ISSN14203049
Volume15Issue:6Pages:4227
AbstractFree and polymeric carbohydrates in Cordyceps, a valued edible mushroom and well-known traditional Chinese medicine, were determined using stepwise pressurized liquid extraction (PLE) extraction and GC-MS. Based on the optimized PLE conditions, acid hydrolysis and derivatization, ten monosaccharides, namely rhamnose, ribose, arabinose, xylose, mannose, glucose, galactose, mannitol, fructose and sorbose in 13 samples of natural and cultured Cordyceps were qualitatively and quantitatively analyzed and compared with myo-inositol hexaacetate as internal standard. The results showed that natural C. sinensis contained more than 7.99% free mannitol and a small amount of glucose, while its polysaccharides were usually composed of mannose, glucose and galactose with a molar ratio of 1.00:16.61∼3.82:1.60∼1.28. However, mannitol in cultured C. sinensis and cultured C. militaris were less than 5.83%, and free glucose was only detected in a few samples, while their polysaccharides were mainly composed of mannose, glucose and galactose with molar ratios of 1.00:3.01∼1.09:3.30∼1.05 and 1.00:2.86∼1.28:1.07∼0.78, respectively. Natural and cultured Cordyceps could be discriminated by hierarchical clustering analysis based on its free carbohydrate contents.
KeywordCarbohydrates Cordyceps GC-MS Polysaccharides Pressurized liquid extraction (PLE)
DOI10.3390/molecules15064227
URLView the original
Language英语
The Source to ArticleScopus
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Cited Times [WOS]:37   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
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GB/T 7714
Guan J.,Yang F.-Q.,Li S.-P.. Evaluation of carbohydrates in natural and cultured Cordyceps by pressurized liquid extraction and gas chromatography coupled with mass spectrometry[J]. Molecules,2010,15(6):4227.
APA Guan J.,Yang F.-Q.,&Li S.-P..(2010).Evaluation of carbohydrates in natural and cultured Cordyceps by pressurized liquid extraction and gas chromatography coupled with mass spectrometry.Molecules,15(6),4227.
MLA Guan J.,et al."Evaluation of carbohydrates in natural and cultured Cordyceps by pressurized liquid extraction and gas chromatography coupled with mass spectrometry".Molecules 15.6(2010):4227.
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