UM  > 中華醫藥研究院
Characterization of metabolism and in vitro permeability study of notoginsenoside R1 from radix notoginseng
Ruan J.-Q.; Leong W.-I.; Yan R.; Wang Y.-T.
2010
Source PublicationJournal of Agricultural and Food Chemistry
ISSN218561
Volume58Issue:9Pages:5770
Abstract

As a main and characteristic constituent in Radix notoginseng, the fate of notoginsenoside R1 (NGR1) in human is largely unknown. The present study investigated, for the first time, NGR1 metabolism by human intestinal bacteria and liver subcellular fractions, and permeability properties of NGR1 and resultant metabolites on a Caco-2 model. Samples were qualitatively analyzed using HPLC-MS/MS and quantitatively determined using HPLC-UV. When incubated with pooled human intestinal bacteria anaerobically, NGR1 showed biphasic elimination: an insignificant decrease in the first 8 h followed by a rapid elimination during 8-48 h. Four metabolites, three unambiguously identified as ginsenosides Rg1, F1 and 20(S)-protopanaxatriol formed via stepwise deglycosylation, and one tentatively assigned as a dehydrogenated protopanaxatriol with transformation occurring at the tetracyclic triterpenoid skeleton, were produced sequentially. Rg1 and F1 were formed transiently at low apparent velocities, while 20(S)-protopanaxatriol was the major metabolite with a formation rate close to the rate of NGR1 elimination and a low elimination rate. NGR1 remained intact in human liver S9 or microsomes over 1 h. Transport study of NGR1 and its metabolites revealed an ascending permeability order with stepwise deglycosylation. Taken together, the results revealed a determinant role of intestinal bacteria in the overall disposition and potential bioactivity of NGR1 in human. © 2010 American Chemical Society.

KeywordCaco-2 Intestinal Bacteria Metabolism Notoginsenoside R1 Permeability Radix Notoginseng
DOIhttp://doi.org/10.1021/jf1005885
URLView the original
Indexed BySCI
Language英语
WOS Research AreaAgriculture ; Chemistry ; Food Science & Technology
WOS SubjectAgriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology
WOS IDWOS:000277237500078
The Source to ArticleScopus
Fulltext Access
Citation statistics
Cited Times [WOS]:47   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorYan R.
AffiliationInstitute of Chinese Medical Sciences, University of Macau, Taipa, Macau SAR, China
First Author AffilicationInstitute of Chinese Medical Sciences
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Ruan J.-Q.,Leong W.-I.,Yan R.,et al. Characterization of metabolism and in vitro permeability study of notoginsenoside R1 from radix notoginseng[J]. Journal of Agricultural and Food Chemistry,2010,58(9):5770.
APA Ruan J.-Q.,Leong W.-I.,Yan R.,&Wang Y.-T..(2010).Characterization of metabolism and in vitro permeability study of notoginsenoside R1 from radix notoginseng.Journal of Agricultural and Food Chemistry,58(9),5770.
MLA Ruan J.-Q.,et al."Characterization of metabolism and in vitro permeability study of notoginsenoside R1 from radix notoginseng".Journal of Agricultural and Food Chemistry 58.9(2010):5770.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Ruan J.-Q.]'s Articles
[Leong W.-I.]'s Articles
[Yan R.]'s Articles
Baidu academic
Similar articles in Baidu academic
[Ruan J.-Q.]'s Articles
[Leong W.-I.]'s Articles
[Yan R.]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Ruan J.-Q.]'s Articles
[Leong W.-I.]'s Articles
[Yan R.]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.