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Effects of sample preparation methods on the quantification of nucleosides in natural and cultured Cordyceps
Yang F.Q.; Li S.P.
2008
Source PublicationJournal of Pharmaceutical and Biomedical Analysis
ISSN7317085
Volume48Issue:1Pages:231
AbstractSample preparation is the first and very important step, which can greatly influence the repeatability and accuracy of the analysis. To date, several sample preparation methods with different solvents have been used for quantitative determination of nucleosides in Cordyceps, but their data are greatly various. In this study, five nucleosides, including adenosine, guanosine, inosine, uridine and cordycepin, in Cordyceps were determined using three extraction methods i.e. organic solvent pressurized liquid extraction, boiling water extraction and ambient temperature water extraction and high performance liquid chromatography (HPLC)-diode array detection (DAD). The similar results were obtained when organic solvent pressurized liquid extraction and boiling water extraction were applied. However, the amounts of nucleosides in natural C. sinensis and cultured C. militaris extracted with ambient temperature water were greatly increased except those of adenosine in natural C. sinensis and cordycepin in cultured C. militaris. In addition, the amount of investigated nucleosides in cultured C. sinensis had no obvious variation among the three extraction methods. The results suggest that sample preparation has significant effect on the quantification of nucleosides in Cordyceps. © 2008 Elsevier B.V. All rights reserved.
KeywordCordyceps Diode array detection (DAD) High performance liquid chromatography (HPLC) Nucleoside Sample preparation
DOI10.1016/j.jpba.2008.05.012
URLView the original
Language英语
The Source to ArticleScopus
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Cited Times [WOS]:41   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Yang F.Q.,Li S.P.. Effects of sample preparation methods on the quantification of nucleosides in natural and cultured Cordyceps[J]. Journal of Pharmaceutical and Biomedical Analysis,2008,48(1):231.
APA Yang F.Q.,&Li S.P..(2008).Effects of sample preparation methods on the quantification of nucleosides in natural and cultured Cordyceps.Journal of Pharmaceutical and Biomedical Analysis,48(1),231.
MLA Yang F.Q.,et al."Effects of sample preparation methods on the quantification of nucleosides in natural and cultured Cordyceps".Journal of Pharmaceutical and Biomedical Analysis 48.1(2008):231.
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