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Qualitative and quantitative analysis of four species of Curcuma rhizomes using twice development thin layer chromatography
Zhang J.S.; Guan J.; Yang F.Q.; Liu H.G.; Cheng X.J.; Li S.P.
2008
Source PublicationJournal of Pharmaceutical and Biomedical Analysis
ISSN7317085
Volume48Issue:3Pages:1024
AbstractThe rhizomes of Curcuma phaeocaulis, Curcuma kwangsiensis, Curcuma wenyujin and Curcuma longa are used as Ezhu or Jianghuang in traditional Chinese medicine for a long time. Due to their similar morphological characters, it is difficult to distinguish their origins of raw materials used in clinic. In this study, a simple, rapid and reliable twice development TLC method was developed for qualitative and quantitative analysis of the four species of Curcuma rhizomes. The chromatography was performed on silica gel 60F254 plate with chloroform-methanol-formic acid (80:4:0.8, v/v/v) and petroleum ether-ethyl acetate (90:10, v/v) as mobile phase for twice development. The TLC markers were colorized with 1% vanillin-H2SO4 solution. The four species of Curcuma were easily discriminated based on their characteristic TLC profiles, and simultaneous quantification of eight compounds, including bisdemethoxycurcumin, demethoxycurcumin, curcumine, curcumenol, curcumol, curdione, furanodienone and curzerene, in Curcuma were also performed densitometrically at λscan = 518 nm and λreference = 800 nm. The investigated compounds had good linearity (r2 > 0.9905) within test ranges. Therefore, the developed TLC method can be used for quality control of Curcuma rhizomes. © 2008 Elsevier B.V. All rights reserved.
KeywordCurcuma Qualitative Quantitative TLC Twice development
DOI10.1016/j.jpba.2008.07.006
URLView the original
Language英语
The Source to ArticleScopus
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Cited Times [WOS]:41   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
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GB/T 7714
Zhang J.S.,Guan J.,Yang F.Q.,et al. Qualitative and quantitative analysis of four species of Curcuma rhizomes using twice development thin layer chromatography[J]. Journal of Pharmaceutical and Biomedical Analysis,2008,48(3):1024.
APA Zhang J.S.,Guan J.,Yang F.Q.,Liu H.G.,Cheng X.J.,&Li S.P..(2008).Qualitative and quantitative analysis of four species of Curcuma rhizomes using twice development thin layer chromatography.Journal of Pharmaceutical and Biomedical Analysis,48(3),1024.
MLA Zhang J.S.,et al."Qualitative and quantitative analysis of four species of Curcuma rhizomes using twice development thin layer chromatography".Journal of Pharmaceutical and Biomedical Analysis 48.3(2008):1024.
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