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Discrimination of polysaccharides from traditional Chinese medicines using saccharide mapping-Enzymatic digestion followed by chromatographic analysis
Guan J.; Li S.P.
2010
Source PublicationJournal of Pharmaceutical and Biomedical Analysis
ISSN7317085
Volume51Issue:3Pages:590
AbstractPolysaccharides isolated from traditional Chinese medicines (TCMs) exhibit multiple pharmacological activities. However, quality control of polysaccharides is a challenge because of their complicate structure and macro-molecular mass. In this study, saccharide mapping based on specific enzymatic digestion of polysaccharides and chromatographic analysis was proposed to discriminate the polysaccharides from different TCMs. Endo-carbohydrase such as glucanase, arabinanase, xylanase, galactanase, cellulase, amylase and pectinase were used for enzymatic digestion of polysaccharides from 9 TCMs namely Panax ginseng, P. notoginseng, P. quinquefolium, Cordyceps sinensis, C. militaris, Ganoderma lucidum, G. sinense, Astragalus membranaceus and Angelica sinensis. By using high performance size exclusion chromatography (HPSEC) as well as derivatization with 1-Phenyl-3-methyl-5-pyrazolone (PMP) and HPLC analysis, the enzymatic hydrolysis properties of polysaccharides and their saccharide mapping were determined. The polysaccharides from 9 TCMs were firstly successfully distinguished based on their characteristic saccharide maps, which is helpful to improve the quality control of polysaccharides. © 2009 Elsevier B.V. All rights reserved.
KeywordChromatographic analysis Enzymatic digestion Polysaccharide Saccharide mapping Traditional Chinese Medicines
DOI10.1016/j.jpba.2009.09.026
URLView the original
Language英语
The Source to ArticleScopus
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Cited Times [WOS]:46   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
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GB/T 7714
Guan J.,Li S.P.. Discrimination of polysaccharides from traditional Chinese medicines using saccharide mapping-Enzymatic digestion followed by chromatographic analysis[J]. Journal of Pharmaceutical and Biomedical Analysis,2010,51(3):590.
APA Guan J.,&Li S.P..(2010).Discrimination of polysaccharides from traditional Chinese medicines using saccharide mapping-Enzymatic digestion followed by chromatographic analysis.Journal of Pharmaceutical and Biomedical Analysis,51(3),590.
MLA Guan J.,et al."Discrimination of polysaccharides from traditional Chinese medicines using saccharide mapping-Enzymatic digestion followed by chromatographic analysis".Journal of Pharmaceutical and Biomedical Analysis 51.3(2010):590.
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