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Anti-tumor potential of ethanol extract of Curcuma phaeocaulis Valeton against breast cancer cells
Chen X.1,2; Pei L.3; Zhong Z.1,2; Guo J.1,2; Zhang Q.1,2; Wang Y.1,2
2011
Source PublicationPhytomedicine
ISSN9447113
Volume18Issue:14Pages:1238
Abstract

Curcuma phaeocaulis Valeton is a commonly prescribed Chinese medical herb for tumor therapy. In this study, an extract of Curcuma phaeocaulis Valeton referred as Cpv was prepared and its anti-tumor effect was evaluated with MCF-7 and MDA-MB-231 cells. Curcuma phaeocaulis Valeton power was extracted with ethanol and the main components of the extract (Cpv) were analyzed with HPLC. The effect of Cpv on MCF-7 cells proliferation, intracellular reactive oxygen species (ROS) formation, mitochondrial membrane potential (ΔΨm), apoptosis, apoptotic related proteins, MDA-MB-231 cell migration, and integrins expression were determined. Furthermore, the effect of Cpv on some key signal transduction molecules was also investigated. Furanodienone, germacrone and furanodiene were identified as the main components of Cpv. Cpv treatment significantly inhibited cell proliferation, increased LDH release, induced intracellular ROS formation, and decreased ΔΨm in a dose-dependent manner in MCF-7 cells. Cpv induced apoptosis without affecting cell migration. Cpv increased protein expression of Bax, PARP, cleaved PARP, caspase-3, 7, JNK1, p-p42/44MAPK, NF-κB, IKKα, IKKβ, decreased protein expression of Bcl-2, Bcl-xL, Bim, Bik, Bad, integrin β5, p42/44MAPK without affecting integrin α5, β1, and p38MAPK protein expression. We concluded that Cpv inhibited MCF-7 cells proliferation by inducing apoptosis mediated by increasing ROS formation, decreasing ΔΨm, regulating Bcl-2 family proteins expression, and activating caspases. Cpv treatment also modulated several signaling transduction pathways. These results might provide some molecular basis for the anti-tumor activity of Curcuma phaeocaulis Valeton. © 2011 Elsevier GmbH.

KeywordApoptosis Curcuma Phaeocaulis Valeton Mcf-7 Cell Proliferation
DOIhttp://doi.org/10.1016/j.phymed.2011.06.017
URLView the original
Indexed BySCI
Language英语
WOS Research AreaPlant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
WOS SubjectPlant Sciences ; Chemistry, Medicinal ; Integrative & Complementary Medicine ; Pharmacology & Pharmacy
WOS IDWOS:000297964700007
The Source to ArticleScopus
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Cited Times [WOS]:43   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorWang Y.
Affiliation1.State Key Laboratory of Quality Research in Chinese Medicine, Macau, China
2.Institute of Chinese Medical Sciences, University of Macau, Macau, China
3.LONGHUA Hospital Shanghai University of TCM, Shanghai, China
First Author AffilicationInstitute of Chinese Medical Sciences
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Chen X.,Pei L.,Zhong Z.,et al. Anti-tumor potential of ethanol extract of Curcuma phaeocaulis Valeton against breast cancer cells[J]. Phytomedicine,2011,18(14):1238.
APA Chen X.,Pei L.,Zhong Z.,Guo J.,Zhang Q.,&Wang Y..(2011).Anti-tumor potential of ethanol extract of Curcuma phaeocaulis Valeton against breast cancer cells.Phytomedicine,18(14),1238.
MLA Chen X.,et al."Anti-tumor potential of ethanol extract of Curcuma phaeocaulis Valeton against breast cancer cells".Phytomedicine 18.14(2011):1238.
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