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Quality evaluation of Cordyceps through simultaneous determination of eleven nucleosides and bases by RP-HPLC
Yu L.1; Zhao J.2; Li S.P.1,2; Fan H.2; Hong M.1; Wang Y.T.2; Zhu Q.1
2006
Source PublicationJournal of Separation Science
ISSN16159306
Volume29Issue:7Pages:953
Abstract

A new RP-HPLC method has been developed for the simultaneous determination of 11 nucleosides and bases, including adenosine, cordycepin, cytidine, guanosine, inosine, thymidine, uridine, cytosine, guanine, thymine, and uracil in Cordyceps. Determination was achieved on a Zorbax 300SB C18 analytical column (4.6 × 250 mm id, 5 mm) using gradient elution with diode-array detection. All calibration curves showed good linearity (r2 >0.9995) within the test ranges. The developed method was simple, rapid, and accurate, and showed good reproducibility for the quantification of 11 nucleosides and bases in natural and cultured Cordyceps with both intra-and inter-day variations of less than 1.8%. Furthermore, hierarchical clustering analysis based on the typical peaks of adenosine, cordycepin, and inosine in HPLC profiles from the 11 tested samples showed that natural and cultured Cordyceps were in different clusters, which could provide a means of discriminating between Cordyceps of different origins. Thus, adenosine, cordycepin, and inosine could be used as markers for quality control of Cordyceps. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

KeywordCordyceps Nucleosides Quality Control Rp-hplc
DOIhttp://doi.org/10.1002/jssc.200600007
URLView the original
Indexed BySCI
Language英语
WOS Research AreaChemistry
WOS SubjectChemistry, Analytical
WOS IDWOS:000237786800004
The Source to ArticleScopus
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Cited Times [WOS]:43   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorLi S.P.
Affiliation1.National Standard Lab for TCM,Nanjing University of TCM,Nanjing, China
2.Institute of Chinese MedicalSciences, University of Macau,Taipa, Macau, China
Corresponding Author AffilicationUniversity of Macau
Recommended Citation
GB/T 7714
Yu L.,Zhao J.,Li S.P.,et al. Quality evaluation of Cordyceps through simultaneous determination of eleven nucleosides and bases by RP-HPLC[J]. Journal of Separation Science,2006,29(7):953.
APA Yu L..,Zhao J..,Li S.P..,Fan H..,Hong M..,...&Zhu Q..(2006).Quality evaluation of Cordyceps through simultaneous determination of eleven nucleosides and bases by RP-HPLC.Journal of Separation Science,29(7),953.
MLA Yu L.,et al."Quality evaluation of Cordyceps through simultaneous determination of eleven nucleosides and bases by RP-HPLC".Journal of Separation Science 29.7(2006):953.
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