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Rapid method for simultaneous determination of flavonoid, saponins and polyacetylenes in Folium Ginseng and Radix Ginseng by pressurized liquid extraction and high-performance liquid chromatography coupled with diode array detection and mass spectrometry
Qian Z.M.; Lu J.; Gao Q.P.; Li S.P.
2009
Source PublicationJournal of Chromatography A
ISSN219673
Volume1216Issue:18Pages:3825
AbstractA rapid pressurized liquid extraction (PLE) and high-performance liquid chromatography coupled with diode array detection and mass spectrometry (HPLC-DAD-MS) method for the simultaneous determination of one flavonoid (panasenoside), nine saponins (ginsenoside Rg1, Re, Rf, Rg2, Rb1, Rc, Rb2, Rb3 and Rd) and two polyacetylenes (panaxydol and panaxynol) in Folium Ginseng and Radix Ginseng was developed. A Prevail C18 rocket column (33 mm × 7 mm, 3.0 μm) and gradient elution were used during the analysis. Flavonoid was quantified at 355 nm, and saponins and polyacetylenes were determined at 203 nm. The chromatographic peaks of 12 investigated compounds in samples were unambiguously identified by compared their UV spectra and/or MS data with the related reference compounds. All calibration curves showed good linearity (r > 0.999) within the test ranges. The intra- and inter-day variations for 12 analytes were less than 1.17% and 2.17%, respectively. The developed method was successfully applied to determine the investigated compounds in 10 samples of Radix Ginseng and Folium Ginseng, respectively. The result showed that PLE combined with rocket column HPLC analysis could provide a rapid method for analysis of compounds in traditional Chinese medicines (TCMs), which is helpful to comprehensive evaluation of quality of Radix Ginseng and Folium Ginseng. © 2009 Elsevier B.V. All rights reserved.
KeywordFlavonoid HPLC Panax ginseng Polyacetylenes Pressurized liquid extraction Saponins
DOI10.1016/j.chroma.2009.02.065
URLView the original
Language英语
The Source to ArticleScopus
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Cited Times [WOS]:39   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
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GB/T 7714
Qian Z.M.,Lu J.,Gao Q.P.,et al. Rapid method for simultaneous determination of flavonoid, saponins and polyacetylenes in Folium Ginseng and Radix Ginseng by pressurized liquid extraction and high-performance liquid chromatography coupled with diode array detection and mass spectrometry[J]. Journal of Chromatography A,2009,1216(18):3825.
APA Qian Z.M.,Lu J.,Gao Q.P.,&Li S.P..(2009).Rapid method for simultaneous determination of flavonoid, saponins and polyacetylenes in Folium Ginseng and Radix Ginseng by pressurized liquid extraction and high-performance liquid chromatography coupled with diode array detection and mass spectrometry.Journal of Chromatography A,1216(18),3825.
MLA Qian Z.M.,et al."Rapid method for simultaneous determination of flavonoid, saponins and polyacetylenes in Folium Ginseng and Radix Ginseng by pressurized liquid extraction and high-performance liquid chromatography coupled with diode array detection and mass spectrometry".Journal of Chromatography A 1216.18(2009):3825.
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