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Formononetin, an isoflavone, relaxes rat isolated aorta through endothelium-dependent and endothelium-independent pathways
Wu J.-H.1; Li Q.1,2; Wu M.-Y.1; Guo D.-J.1; Chen H.-L.1; Chen S.-L.1,2; Seto S.-W.3; Au A.L.S.3; Poon C.C.W.3; Leung G.P.H.4; Lee S.M.Y.5; Kwan Y.-W.3; Chan S.-W.1,6
2010
Source PublicationJournal of Nutritional Biochemistry
ISSN9552863
Volume21Issue:7Pages:613
Abstract

We evaluated the vasorelaxation effects of formononetin, an isoflavone/phytoestrogen found abundantly in Astragalus mongholicus Bunge, on rat isolated aorta and the underlying mechanisms involved. Cumulative administration of formononetin, genistein, daidzein and biochanin A relaxed phenylephrine-preconstricted aorta. Formononetin and biochanin A caused a similar magnitude of relaxation whereas daidzein was least potent. Mechanical removal of endothelium, L-NAME (100 μM) and methylene blue (10 μM) suppressed formononetin-induced relaxation. Formononetin increased endothelial nitric oxide (NO) synthase (eNOS), but not inducible NO synthase, activity with an up-regulation of eNOS mRNA and p-eNOSSer1177 protein expression. In endothelium-denuded preparations, formononetin-induced vasorelaxation was significantly reduced by glibenclamide (3 μM) and iberiotoxin (100 nM), and a combination of glibenclamide (3 μM) plus iberiotoxin (100 nM) abolished the relaxation. In contrast, formononetin-elicited endothelium-independent relaxation was not altered by ICI 182,780 (10 μM, an estrogen receptor (ERα/ERβ) antagonist) or mifepristone (10 μM, a progesterone receptor antagonist). In single aortic smooth muscle cells, formononetin caused opening of iberiotoxin-sensitive Ca2+-activated K+ (BKCa) channels and glibenclamide-sensitive adenosine triphosphate (ATP)-dependent K+ (KATP) channels. Thus, our results suggest that formononetin caused vascular relaxation via endothelium/NO-dependent mechanism and endothelium-independent mechanism which involves the activation of BKCa and KATP channels. © 2010 Elsevier Inc.

KeywordBkca And Katp Channels Formononetin Nitric Oxide Vasorelaxation
DOI10.1016/j.jnutbio.2009.03.010
URLView the original
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Nutrition & Dietetics
WOS SubjectBiochemistry & Molecular Biology ; Nutrition & Dietetics
WOS IDWOS:000279363200007
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被引频次[WOS]:49   [WOS记录]     [WOS相关记录]
Document TypeJournal article
专题Institute of Chinese Medical Sciences
Corresponding AuthorChan S.-W.
Affiliation1.State Key Laboratory of Chinese Medicine and Molecular Pharmacology, Shenzhen 518057, PR China
2.Institute of Medicinal Plant Development, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100094, PR China
3.Department of Pharmacology, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong SAR, PR China
4.Department of Pharmacology, Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, PR China
5.Institute of Chinese Medical Sciences, University of Macau, Av. Padre Tomas Pereira S.J., Taipa, Macau, PR China
6.Open Laboratory of Chirotechnology, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hong Kong SAR, PR China
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Wu J.-H.,Li Q.,Wu M.-Y.,et al. Formononetin, an isoflavone, relaxes rat isolated aorta through endothelium-dependent and endothelium-independent pathways[J]. Journal of Nutritional Biochemistry,2010,21(7):613.
APA Wu J.-H..,Li Q..,Wu M.-Y..,Guo D.-J..,Chen H.-L..,...&Chan S.-W..(2010).Formononetin, an isoflavone, relaxes rat isolated aorta through endothelium-dependent and endothelium-independent pathways.Journal of Nutritional Biochemistry,21(7),613.
MLA Wu J.-H.,et al."Formononetin, an isoflavone, relaxes rat isolated aorta through endothelium-dependent and endothelium-independent pathways".Journal of Nutritional Biochemistry 21.7(2010):613.
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