UM  > 中華醫藥研究院
In vitro and in vivo anticancer activity of a novel puerarin nanosuspension against colon cancer, with high efficacy and low toxicity
Wang Y.1; Ma Y.1; Zheng Y.2; Song J.2; Yang X.2; Bi C.2; Zhang D.3; Zhang Q.4
2013
Source PublicationInternational Journal of Pharmaceutics
ISSN3785173
Volume441Issue:2018-01-02Pages:728
Abstract

The present study aims to evaluate the anticancer activity of puerarin nanosuspensions in human colon cancer HT-29 cell line in vitro and in vivo. Puerarin nanosuspensions were prepared by the high-pressure homogenization (HPH) technique. The HT-29 cells were incubated with increasing concentrations of puerarin solution and nanosuspensions for indicated times. MTT evaluated cellular viability and investigated the effect of puerarin on cell proliferation of HT-29. Annexin V-FITC/PI staining method was conducted to determine the influences of the puerarin nanosuspensions on cell cycle and apoptosis. The in vivo anticancer activity of the puerarin nanosuspensions was observed in HT-29 cancer bearing mice. The puerarin nanosuspensions were well re-dispersed in aqueous media a mean diameter about 400-500 nm. Cytotoxicity assay, observation of morphological changes and early apoptosis revealed that the puerarin nanosuspensions could significantly enhance the in vitro anti-proliferation against HT-29 cells compared to the puerarin free solution. The prepared puerarin nanosuspensions in vivo evaluation showed higher anticancer efficacy and lower toxicity compared to the free solution, as shown by changes in tumor volumes, body weights, and survival rates. Based on these data, the potential of the puerarin nanosuspensions to serve as a cancer chemotherapeutic agent for colon cancer could be suggested. © 2012 Elsevier B.V. All rights reserved.

KeywordApoptosis Ht-29 Cells In Vivo Nanosuspensions Puerarin
DOI10.1016/j.ijpharm.2012.10.021
URLView the original
Indexed BySCI
Language英语
WOS Research AreaPharmacology & Pharmacy
WOS SubjectPharmacology & Pharmacy
WOS IDWOS:000314054200087
The Source to ArticleScopus
全文获取链接
引用统计
被引频次[WOS]:61   [WOS记录]     [WOS相关记录]
Document TypeJournal article
专题Institute of Chinese Medical Sciences
Affiliation1.School of Chemistry and Pharmaceutical Engineering, Shandong Polytechnic University, Jinan 250353, China
2.Institute of Chinese Medical Sciences, University of Macau, Macao
3.College of Pharmacy, Shandong University, Jinan 250012, PR China
4.School of Pharmaceutical Sciences, Peking University, Beijing 100083, PR China
推荐引用方式
GB/T 7714
Wang Y.,Ma Y.,Zheng Y.,et al. In vitro and in vivo anticancer activity of a novel puerarin nanosuspension against colon cancer, with high efficacy and low toxicity[J]. International Journal of Pharmaceutics,2013,441(2018-01-02):728.
APA Wang Y..,Ma Y..,Zheng Y..,Song J..,Yang X..,...&Zhang Q..(2013).In vitro and in vivo anticancer activity of a novel puerarin nanosuspension against colon cancer, with high efficacy and low toxicity.International Journal of Pharmaceutics,441(2018-01-02),728.
MLA Wang Y.,et al."In vitro and in vivo anticancer activity of a novel puerarin nanosuspension against colon cancer, with high efficacy and low toxicity".International Journal of Pharmaceutics 441.2018-01-02(2013):728.
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
Google Scholar
中相似的文章 Google Scholar
[Wang Y.]的文章
[Ma Y.]的文章
[Zheng Y.]的文章
Baidu academic
中相似的文章 Baidu academic
[Wang Y.]的文章
[Ma Y.]的文章
[Zheng Y.]的文章
Bing Scholar
中相似的文章 Bing Scholar
[Wang Y.]的文章
[Ma Y.]的文章
[Zheng Y.]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。