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Characterization of gene expression regulated by American ginseng and ginsenoside Rg3 in human colorectal cancer cells
Luo X.2,3,4; Wang C.-Z.1; Chen J.2,3,4; Song W.-X.2; Luo J.2,3,4; Tang N.2,3,4; He B.-C.3,4; Kang Q.3,4; Wang Y.5; Du W.6; He T.-C.2,3,4; Yuan C.-S.1,7
2008
Source PublicationInternational Journal of Oncology
ISSN10196439
Volume32Issue:5Pages:975-983
Abstract

American ginseng (Panax quinquefolius L., Araliaceae) possesses anti-cancer potential and is one of the most commonly used herbal medicines in the United States. Ginsenoside Rg3, one of the saponins in American ginseng, has been shown to inhibit tumor growth. In this study, we sought to characterize the downstream genes targeted by American ginseng extracts in HCT-116 human colorectal cancer cells. We first demonstrated that the content of Rg3 in American ginseng steamed at 120°C for 2 h (referred to as S2h) was significantly increased when compared with that of the unsteamed ginseng. Both S2h and Rg3 exhibited anti-proliferative effects on HCT-116 cells. Using the Affymetrix high density genechips containing more than 40,000 genes and ESTs, the gene expression profiling of HCT-116 cells were assayed. Microarray data indicated that the expression levels of 76 genes were changed significantly after treatment with S2h or Rg3, whereby it was found that 52 of the 76 genes were up-regulated while the remaining 24 were down-regulated. Ingenuity Pathways Analysis of top functions affected by both S2h and Rg3 were carried out. The most effected pathway is the Ephrin receptor pathway. To validate the microarray data, quantitative real-time PCR of six candidate target genes was conducted, whereby it was found that three genes were up-regulated (AKAPA8L, PMPCB and PDE5A) and three were down-regulated (PITPNA, DUS2L and RIC8A). Although further studies are needed to elucidate the mechanisms of action, our findings should expand the understanding of the molecular framework of American ginseng as an anti-cancer agent.

KeywordGene Expression Ginsenoside Rg3 Human Colorectal Cancer Microarray Analysis Panax Quinquefolius
DOIhttps://doi.org/10.3892/ijo.32.5.975
URLView the original
Language英语
WOS Research AreaOncology
WOS SubjectOncology
WOS IDWOS:000255319300002
The Source to ArticleScopus
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Cited Times [WOS]:64   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorYuan C.-S.
Affiliation1.Univ Chicago, Med Ctr, Tang Ctr Herbal Med Res, Chicago, IL 60637 USA
2.Univ Chicago, Dept Surg, Mol Oncol Lab, Chicago, IL 60637 USA
3.Chongqing Med Univ, Chinese Minist Educ, Key Lab Diagnost Med Designated, Chongqing, Peoples R China
4.Chongqing Med Univ, Affiliated Childrens Hosp, Chongqing, Peoples R China
5.Univ Macau, Inst Chinese Med Sci, Taipa, Macau, Peoples R China
6.Univ Chicago, Ben May Dept Canc Res, Chicago, IL 60637 USA
7.Univ Chicago, Med Ctr, Dept Anesthesia & Crit Care, Chicago, IL 60637 USA
Recommended Citation
GB/T 7714
Luo X.,Wang C.-Z.,Chen J.,et al. Characterization of gene expression regulated by American ginseng and ginsenoside Rg3 in human colorectal cancer cells[J]. International Journal of Oncology,2008,32(5):975-983.
APA Luo X..,Wang C.-Z..,Chen J..,Song W.-X..,Luo J..,...&Yuan C.-S..(2008).Characterization of gene expression regulated by American ginseng and ginsenoside Rg3 in human colorectal cancer cells.International Journal of Oncology,32(5),975-983.
MLA Luo X.,et al."Characterization of gene expression regulated by American ginseng and ginsenoside Rg3 in human colorectal cancer cells".International Journal of Oncology 32.5(2008):975-983.
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