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Simultaneous determination of 11 saponins in Panax notoginseng using HPLC-ELSD and pressurized liquid extraction
Wan J.-B.1; Li P.1; Li S.1; Wang Y.1; Dong T.T.-X.2; Tsim K.W.-K.2
2006
Source PublicationJournal of Separation Science
ISSN16159306
Volume29Issue:14Pages:2190
Abstract

A new HPLC coupled with evaporative light scattering detection (ELSD) method was developed for simultaneous determination of 11 major triterpene saponins, namely notoginsenoside R1 (1), ginsenosides Rg1 (2), Re (3), Rf (4), Rb1 (5), Rg2 (6), Rc (7), Rb2 (8), Rb3 (9), Rd (10), and Rg3 (11) in Panax notoginseng, a commonly used traditional Chinese medicine (TCM). Pressurized liquid extraction (PLE) was employed for sample preparation, and the analysis was achieved using a Zorbax ODS C18 column eluted with gradient water-ACN in 60 min. The drift tube temperature of ELSD was set at 60°C, and nitrogen flowrate was at 1.4 L/ min. The method provided good repeatability and sensitivity for quantification of 11 saponins with overall precision (including intra- and interday) and LOD of less than 2.9% (RSD) and 98 ng, respectively. The validated method was successfully applied to quantify 11 saponins in 28 samples of P. notoginseng collected in different places, which is helpful to control the quality of P. notoginseng and its related products. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

KeywordGinsenoside Hplc-elsd Notoginsenoside Panax Notoginseng Pressurized Liquid Extraction
DOI10.1002/jssc.200600103
URLView the original
Indexed BySCI
Language英语
WOS Research AreaChemistry
WOS SubjectChemistry, Analytical
WOS IDWOS:000240891500010
The Source to ArticleScopus
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Cited Times [WOS]:50   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorLi S.
Affiliation1.Institute of Chinese Medical Sciences, University of Macau, Taipa, Macau, P. R. China.
2.Department of Biology and Biotechnology Research Institute, The Hong Kong University of Science and Technology, Hong Kong, P. R. China
First Author AffilicationInstitute of Chinese Medical Sciences
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Wan J.-B.,Li P.,Li S.,et al. Simultaneous determination of 11 saponins in Panax notoginseng using HPLC-ELSD and pressurized liquid extraction[J]. Journal of Separation Science,2006,29(14):2190.
APA Wan J.-B.,Li P.,Li S.,Wang Y.,Dong T.T.-X.,&Tsim K.W.-K..(2006).Simultaneous determination of 11 saponins in Panax notoginseng using HPLC-ELSD and pressurized liquid extraction.Journal of Separation Science,29(14),2190.
MLA Wan J.-B.,et al."Simultaneous determination of 11 saponins in Panax notoginseng using HPLC-ELSD and pressurized liquid extraction".Journal of Separation Science 29.14(2006):2190.
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