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Ganoderic acid DM, a natural triterpenoid, induces DNA damage, G1 cell cycle arrest and apoptosis in human breast cancer cells
Wu G.-S.1,2; Lu J.-J.1,2; Guo J.-J.1,2; Li Y.-B.1,2; Tan W.1,2; Dang Y.-Y.1,2; Zhong Z.-F.1,2; Xu Z.-T.1,2; Chen X.-P.1,2; Wang Y.-T.1,2
2012
Source PublicationFitoterapia
ISSN0367326X
Volume83Issue:2Pages:408
Abstract

Ganoderic acid DM (GADM) is a triterpenoid isolated from Ganoderma lucidum, a well-known edible medicinal mushroom. In the present study, we found that GADM effectively inhibited cell proliferation and colony formation in MCF-7 human breast cancer cells, which was much stronger than that of MDA-MB-231 breast cancer cells. GADM both concentration- and time-dependently mediated G1 cell cycle arrest and significantly decreased the protein level of CDK2, CDK6, cycle D1, p-Rb and c-Myc in MCF-7 cells. Moreover, GADM obviously induced DNA fragmentation and cleavage of PARP which are the characteristics of apoptosis and decreased the mitochondrial membrane potential in MCF-7 cells. Besides, we also showed that GADM elicited DNA damage as measured by comet assay which is a sensitive method for DNA damage detection. γ-H2AX, a marker of DNA damage, was also slightly up-regulated after treated with GADM for 6 h, suggesting that the G1 cell cycle arrest and apoptosis induced by GADM may be partially resulted from GADM-induced DNA damage. These results have advanced our current understandings of the anti-cancer mechanisms of GADM. © 2011 Elsevier B.V. All rights reserve.

KeywordApoptosis Dna Damage G1 Cell Cycle Arrest Ganoderic Acid Dm Mcf-7
DOIhttp://doi.org/10.1016/j.fitote.2011.12.004
URLView the original
Indexed BySCI
Language英语
WOS Research AreaPharmacology & Pharmacy
WOS SubjectChemistry, Medicinal ; Pharmacology & Pharmacy
WOS IDWOS:000300817000025
The Source to ArticleScopus
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Cited Times [WOS]:66   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorWang Y.-T.
Affiliation1.State Key Laboratory of Quality Research in Chinese Medicine (University of Macau), Macao, 999078, PR China
2.Institute of Chinese Medical Sciences, University of Macau, Macao, 999078, PR China
First Author AffilicationUniversity of Macau;  Institute of Chinese Medical Sciences
Corresponding Author AffilicationUniversity of Macau;  Institute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Wu G.-S.,Lu J.-J.,Guo J.-J.,et al. Ganoderic acid DM, a natural triterpenoid, induces DNA damage, G1 cell cycle arrest and apoptosis in human breast cancer cells[J]. Fitoterapia,2012,83(2):408.
APA Wu G.-S..,Lu J.-J..,Guo J.-J..,Li Y.-B..,Tan W..,...&Wang Y.-T..(2012).Ganoderic acid DM, a natural triterpenoid, induces DNA damage, G1 cell cycle arrest and apoptosis in human breast cancer cells.Fitoterapia,83(2),408.
MLA Wu G.-S.,et al."Ganoderic acid DM, a natural triterpenoid, induces DNA damage, G1 cell cycle arrest and apoptosis in human breast cancer cells".Fitoterapia 83.2(2012):408.
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