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Inhibition of three selected beverage extracts on α-glucosidase and rapid identification of their active compounds using HPLC-DAD-MS/MS and biochemical detection
Li D.-Q.; Qian Z.-M.; Li S.-P.
2010
Source PublicationJournal of Agricultural and Food Chemistry
ISSN218561
Volume58Issue:11Pages:6608
AbstractInhibition of α-glucosidase is a therapeutic approach for diabetes. In this study, a method based on online liquid chromatography-diode array detection-tandem mass spectrometry and biochemical detection (LC-DAD-MS/MS-BCD) was developed to screen and identify α-glucosidase inhibitors from selected beverage extracts, including pu-erh tea (Camellia sinensis var. assamica), eagle tea (Litsea coreana Levl.), and radix glycyrrhizae (Glycyrrhiza uralensis Fisch.). As a result, two components, (-)-epigallocatechingallate (EGCG) and (-)-epicatechingallate (ECG), as potent α-glucosidase inhibitors, were found in pu-erh tea. The IC50 values of EGCG and ECG on α-glucosidase (EC 3.2.1.20, from Saccharomyces cerevisiae) were 175.1 and 246.9 μM, respectively, and both were lower than that of acarbose (IC50 = 3553.0 μM), a commercial α-glucosidase inhibitor. Kinetic studies revealed that both EGCG and ECG inhibited α-glucosidase activity in a noncompetitive manner. The study suggests that the developed LC-DAD-MS/MS-BCD system is a powerful tool for rapid screening and identification of α-glucosidase inhibitors in complex samples and that EGCG and ECG may be good candidates as α-glucosidase inhibitors. © 2010 American Chemical Society.
KeywordBiochemical detection Eagle tea HPLC Pu-erh tea R-glucosidase Radix glycyrrhizae
DOI10.1021/jf100853c
URLView the original
Language英语
The Source to ArticleScopus
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Cited Times [WOS]:61   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
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GB/T 7714
Li D.-Q.,Qian Z.-M.,Li S.-P.. Inhibition of three selected beverage extracts on α-glucosidase and rapid identification of their active compounds using HPLC-DAD-MS/MS and biochemical detection[J]. Journal of Agricultural and Food Chemistry,2010,58(11):6608.
APA Li D.-Q.,Qian Z.-M.,&Li S.-P..(2010).Inhibition of three selected beverage extracts on α-glucosidase and rapid identification of their active compounds using HPLC-DAD-MS/MS and biochemical detection.Journal of Agricultural and Food Chemistry,58(11),6608.
MLA Li D.-Q.,et al."Inhibition of three selected beverage extracts on α-glucosidase and rapid identification of their active compounds using HPLC-DAD-MS/MS and biochemical detection".Journal of Agricultural and Food Chemistry 58.11(2010):6608.
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