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The angiogenic effects of Angelica sinensis extract on HUVEC in vitro and zebrafish in vivo
Lam H.-W.1; Lin H.-C.1,2; Lao S.-C.1; Gao J.-L.1; Hong S.-J.1; Leong C.-W.1; Yue P.Y.-K.3; Kwan Y.-W.4; Leung A.Y.-H.5; Wang Y.-T.1; Lee S.M.-Y.1,6
2008
Source PublicationJournal of Cellular Biochemistry
ISSN7302312
Volume103Issue:1Pages:195
Abstract

Angiogenesis plays an important role in a wide range of physiological processes such as wound healing and fetal development. Many diseases are associated with imbalances in regulation of angiogenesis, in which it is either excessive or there is insufficient blood vessel formation. Angelica sinensis (AS), commonly used in the prescriptions of Chinese medicine, is a potential candidate for curing such diseases. However, biological effects of AS on angiogenesis and underlying mechanisms are yet to be fully elucidated. This investigation describes the angiogenic effects of AS extract on human endothelial cells (HUVEC) in vitro and zebrafish in vivo. The extract was demonstrated, by XTT assay and microscopic cell counting, to stimulate the proliferation of HUVEC; in addition, flow cytometry analysis indicated that the extract increased the percentage of HUVEC in the S phase. The wound healing migration assay illustrated that a dramatic increase in migration could be measured in AS extract-treated HUVEC. Meanwhile, the number of invaded cells and the mean tube length were significantly increased in AS extract treatment groups. The extract was also demonstrated to promote changes in subintestinal vessels (SIVs) in zebrafish, one feature of angiogenesis. In addition, AS extract was found by real-time PCR to enhance vascular endothelial growth factor (VEGF) mRNA expression. In a bead-based immunoassay, higher levels of p38 and JN K1/2 expression were also observed in effusions compared with control cells. All results suggest that Angelica sinensis extract can promote angiogenesis, and that the angiogenic effects involve p38 and JNK 1/2 phosphorylation. © 2007 Wiley-Liss, Inc.

KeywordAngelica Sinensis Angiogenesis Huvec Vegf Zebrafish
DOIhttp://doi.org/10.1002/jcb.21403
URLView the original
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Cell Biology
WOS SubjectBiochemistry & Molecular Biology ; Cell Biology
WOS IDWOS:000252334400016
The Source to ArticleScopus
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Cited Times [WOS]:62   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorWang Y.-T.; Lee S.M.-Y.
Affiliation1.Institute of Chinese Medical Sciences, University of Macau, Av. Padre Toma ́s Pereira S.J., Taipa,Macao, China
2.Institute of Clinical Pharmacology, Guangzhou University of Chinese Medicine, Guangzhou, China
3.Department of Biology, Hong Kong Baptist University, Hong Kong, China
4.Department of Pharmacology, The Chinese University of Hong Kong, Hong Kong, China
5.Department of Medicine, University of Hong Kong, Hong Kong, China
6.Institute of Chinese Medicine, The Chinese University of Hong Kong, Hong Kong, China
First Author AffilicationInstitute of Chinese Medical Sciences
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Lam H.-W.,Lin H.-C.,Lao S.-C.,et al. The angiogenic effects of Angelica sinensis extract on HUVEC in vitro and zebrafish in vivo[J]. Journal of Cellular Biochemistry,2008,103(1):195.
APA Lam H.-W..,Lin H.-C..,Lao S.-C..,Gao J.-L..,Hong S.-J..,...&Lee S.M.-Y..(2008).The angiogenic effects of Angelica sinensis extract on HUVEC in vitro and zebrafish in vivo.Journal of Cellular Biochemistry,103(1),195.
MLA Lam H.-W.,et al."The angiogenic effects of Angelica sinensis extract on HUVEC in vitro and zebrafish in vivo".Journal of Cellular Biochemistry 103.1(2008):195.
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