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Label-free detection of sub-nanomolar lead(II) ions in aqueous solution using a metal-based luminescent switch-on probe
He H.-Z.; Leung K.-H.; Yang H.; Shiu-Hin Chan D.; Leung C.-H.; Zhou J.; Bourdoncle A.; Mergny J.-L.; Ma D.-L.
2013
Source PublicationBiosensors and Bioelectronics
ISSN9565663
Volume41Issue:1Pages:871
AbstractA label-free oligonucleotide-based luminescent switch-on assay has been developed for the selective detection of sub-nanomolar Pb2+ ions in aqueous solution and real water samples. An iridium(III) complex was employed as a G-quadruplex specific luminescent probe and a guanine rich DNA (PS2.M, 5'-GTG3TAG3CG3T2G2-3') was employed as recognition unit for Pb2+ ions. The PS2.M exists in a single-stranded conformation in the absence of Pb2+ ions, and the weak binding of the iridium(III) probe to ssDNA results in a weak luminescence signal. Upon binding to Pb2+ ions, the single-stranded DNA sequence (PS2.M) is induced into a G-quadruplex conformation, which greatly enhances the luminescence emission of the iridium(III) probe. The assay can detect Pb2+ ions in aqueous media with a limit of detection of 600pM. It also exhibits good selectivity for Pb2+ ions over other heavy metal ions. Furthermore, the application of the assay for the detection of Pb2+ ions in spiked river water samples has been demonstrated. © 2012 Elsevier B.V.
KeywordG-quadruplex Iridium(III) complex Label-free detection Lead(II) ion Luminescent probe
DOI10.1016/j.bios.2012.08.060
URLView the original
Language英语
The Source to ArticleScopus
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Cited Times [WOS]:71   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
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GB/T 7714
He H.-Z.,Leung K.-H.,Yang H.,et al. Label-free detection of sub-nanomolar lead(II) ions in aqueous solution using a metal-based luminescent switch-on probe[J]. Biosensors and Bioelectronics,2013,41(1):871.
APA He H.-Z..,Leung K.-H..,Yang H..,Shiu-Hin Chan D..,Leung C.-H..,...&Ma D.-L..(2013).Label-free detection of sub-nanomolar lead(II) ions in aqueous solution using a metal-based luminescent switch-on probe.Biosensors and Bioelectronics,41(1),871.
MLA He H.-Z.,et al."Label-free detection of sub-nanomolar lead(II) ions in aqueous solution using a metal-based luminescent switch-on probe".Biosensors and Bioelectronics 41.1(2013):871.
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