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Saikosaponin a and its epimer saikosaponin d exhibit anti-inflammatory activity by suppressing activation of NF-κB signaling pathway
Lu C.-N.1,3; Yuan Z.-G.2,3; Zhang X.-L.1,3; Yan R.4; Zhao Y.-Q.1,3; Liao M.2,3; Chen J.-X.1,3
2012
Source PublicationInternational Immunopharmacology
ISSN15675769
Volume14Issue:1Pages:121
Abstract

Saikosaponin a (SSa) and its epimer saikosaponin d (SSd) are major triterpenoid saponin derivatives from Radix bupleuri (RB), which has been long used in Chinese traditional medicine for treatment of various inflammation-related diseases. In the present study, the anti-inflammatory activity, as well as the underlying mechanism, of SSa and SSd was investigated in lipopolysaccharide (LPS)-induced RAW264.7 cells. Our results demonstrated that both SSa and SSd significantly inhibited the expression of inducible nitric-oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in LPS-induced RAW264.7 cells, and finally resulted in the reduction of nitric oxide (NO) and prostaglandin E2 (PGE2). In addition, LPS-induced production of major pro-inflammatory cytokines: the tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), was suppressed in a dose-dependent manner by the treatment of SSa or SSd in RAW264.7 cells. Further analysis revealed that both SSa and SSd could inhibit translocation of nuclear factor-κB (NF-κB) from the cytoplasm to the nucleus in the LPS-induced RAW264.7 cells. Furthermore, SSa and SSd exhibited significant anti-inflammatory activity in two different murine models of acute inflammation, carrageenan-induced paw edema in rats and acetic acid-induced vascular permeability in mice. In conclusion, SSa and SSd showed potent anti-inflammatory activity through inhibitory effects on NF-κB activation and thereby on iNOS, COX-2 and pro-inflammatory cytokines. © 2012 Elsevier B.V.

KeywordInflammation Nf-κb Raw264.7 Cell Saikosaponin a Saikosaponin d
DOIhttps://doi.org/10.1016/j.intimp.2012.06.010
URLView the original
Indexed BySCI
Language英语
WOS Research AreaImmunology ; Pharmacology & Pharmacy
WOS SubjectImmunology ; Pharmacology & Pharmacy
WOS IDWOS:000307693200017
The Source to ArticleScopus
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Cited Times [WOS]:87   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.The Guangdong Provincial Key Laboratory of Veterinary Drugs Development and Safety Evaluation, South China Agricultural University, Guangzhou 510642, China
2.The Guangdong Provincial Key Laboratory of Zoonoses Control and Prevention, South China Agricultural University, Guangzhou 510642, China
3.College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China
4.State Key Laboratory of Quality Research in Chinese Medicine Institute of Chinese Medical Sciences, University of Macau, Macau 999078, China
Recommended Citation
GB/T 7714
Lu C.-N.,Yuan Z.-G.,Zhang X.-L.,et al. Saikosaponin a and its epimer saikosaponin d exhibit anti-inflammatory activity by suppressing activation of NF-κB signaling pathway[J]. International Immunopharmacology,2012,14(1):121.
APA Lu C.-N..,Yuan Z.-G..,Zhang X.-L..,Yan R..,Zhao Y.-Q..,...&Chen J.-X..(2012).Saikosaponin a and its epimer saikosaponin d exhibit anti-inflammatory activity by suppressing activation of NF-κB signaling pathway.International Immunopharmacology,14(1),121.
MLA Lu C.-N.,et al."Saikosaponin a and its epimer saikosaponin d exhibit anti-inflammatory activity by suppressing activation of NF-κB signaling pathway".International Immunopharmacology 14.1(2012):121.
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