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Simultaneous determination of 15 flavonoids in Epimedium using pressurized liquid extraction and high-performance liquid chromatography
Chen X.J.1,2; Guo B.L.3; Li S.P.1; Zhang Q.W.1; Tu P.F.2; Wang Y.T.1
2007
Source PublicationJournal of Chromatography A
ISSN219673
Volume1163Issue:2018-01-02Pages:96
Abstract

Herba Epimedii (family Berberidaceae), Yinyanghuo in Chinese, is one of the commonly used Chinese medicines. Flavonoids are considered as its active components. In this study, a reliable pressurized liquid extraction (PLE) and HPLC method was developed for simultaneous determination of 15 flavonoids, namely hexandraside E, kaempferol-3-O-rhamnoside, hexandraside F, epimedin A, epimedin B, epimedin C, icariin, epimedoside C, baohuoside II, caohuoside C, baohuoside VII, sagittatoside A, sagittatoside B, 2″-O-rhamnosyl icariside II and baohuoside I in different species of Epimedium. The analysis was performed by using a Zorbax SB-C18 analytical column (250 mm × 4.6 mm I.D., 5 μm) at gradient elution of water and acetonitrile with diode-array detection (270 nm). All calibration curves showed good linearity (r2 > 0.9997) within test ranges. The LOD and LOQ were lower than 1.31 ng and 2.62 ng on column, respectively. The RSD for intra- and inter-day of 15 analytes was less than 3.8% at three levels, and the recoveries were 90.5-106.8%. The validated method was successfully applied for the analysis of 15 flavonoids in different species of Epimedium which had great variation on the contents of investigated flavonoids. Hierarchical clustering analysis based on the characteristics of 15 investigated compound peaks in HPLC profiles showed that 26 samples were divided into three main clusters, which were in accordance with their flavonoid contents. Four flavonoids including epimedin A, B, C and icariin were optimized as markers for quality control of the species of Epimedium used as Yinyanghuo. © 2007 Elsevier B.V. All rights reserved.

KeywordEpimedium Flavonoids Hplc Pressurized Liquid Extraction
DOIhttp://doi.org/10.1016/j.chroma.2007.06.020
URLView the original
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000249310800009
The Source to ArticleScopus
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Cited Times [WOS]:67   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorLi S.P.; Wang Y.T.
Affiliation1.Institute of Chinese Medical Sciences, University of Macau, Taipa, Macao SAR, China
2.Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University, Beijing 100083, China
3.Institute of Medicinal Plants Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100094, China
First Author AffilicationInstitute of Chinese Medical Sciences
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Chen X.J.,Guo B.L.,Li S.P.,et al. Simultaneous determination of 15 flavonoids in Epimedium using pressurized liquid extraction and high-performance liquid chromatography[J]. Journal of Chromatography A,2007,1163(2018-01-02):96.
APA Chen X.J.,Guo B.L.,Li S.P.,Zhang Q.W.,Tu P.F.,&Wang Y.T..(2007).Simultaneous determination of 15 flavonoids in Epimedium using pressurized liquid extraction and high-performance liquid chromatography.Journal of Chromatography A,1163(2018-01-02),96.
MLA Chen X.J.,et al."Simultaneous determination of 15 flavonoids in Epimedium using pressurized liquid extraction and high-performance liquid chromatography".Journal of Chromatography A 1163.2018-01-02(2007):96.
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