UM  > 中華醫藥研究院
Qualitative and quantitative determination of nucleosides, bases and their analogues in natural and cultured Cordyceps by pressurized liquid extraction and high performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS)
Fan H.1,2; Li S.P.1; Xiang J.J.2; Lai C.M.1; Yang F.Q.1; Gao J.L.1; Wang Y.T.1
2006
Source PublicationAnalytica Chimica Acta
ISSN32670
Volume567Issue:2Pages:218
Abstract

A simple and rapid pressurized liquid extraction (PLE) and high performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS) method was developed for qualitative and quantitative determination of nucleosides, bases and their analogues in natural and cultured Cordyceps. The samples were extracted using PLE. The separation was achieved on a ZORBAX Eclipse XDB-C18 column with gradient elution of methanol and 5 mM aqueous ammonium acetate as mobile phase. Target compounds were identified by characterizing their product ions, precursor ions and retention times. Quantitative analysis of investigated compounds were performed using time programmed selective ion monitoring (SIM) or selective reaction monitoring (SRM) with 10 segments in positive (negative for uridine) ion mode. The results showed that 43 bases, nucleosides and their analogues were detected in Cordyceps, of these 16 compounds were identified. The simultaneous determination of seven nucleosides and six bases in Cordyceps was achieved using PLE and HPLC-ESI-MS/MS method described above, which afforded good linearity, selectivity, precision, recovery, short analysis time as well as LOD and LOQ in the ng/ml range. © 2006 Elsevier B.V. All rights reserved.

KeywordBases Cordyceps Hplc-esi-ms/ms Nucleosides Pressurized Liquid Extraction Qualitative And Quantitative Determination
DOI10.1016/j.aca.2006.03.032
URLView the original
Indexed BySCI
Language英语
WOS Research AreaChemistry
WOS SubjectChemistry, Analytical
WOS IDWOS:000237990500011
The Source to ArticleScopus
Fulltext Access
Citation statistics
Cited Times [WOS]:84   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorLi S.P.
Affiliation1.Institute of Chinese Medical Sciences, University of Macau, Taipa, Macau SAR, China
2.Laboratory of Molecular Immunology and Antibody Engineering, Life Science and Technological College, Jinan University, Guangzhou, China
First Author AffilicationInstitute of Chinese Medical Sciences
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Fan H.,Li S.P.,Xiang J.J.,et al. Qualitative and quantitative determination of nucleosides, bases and their analogues in natural and cultured Cordyceps by pressurized liquid extraction and high performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS)[J]. Analytica Chimica Acta,2006,567(2):218.
APA Fan H..,Li S.P..,Xiang J.J..,Lai C.M..,Yang F.Q..,...&Wang Y.T..(2006).Qualitative and quantitative determination of nucleosides, bases and their analogues in natural and cultured Cordyceps by pressurized liquid extraction and high performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS).Analytica Chimica Acta,567(2),218.
MLA Fan H.,et al."Qualitative and quantitative determination of nucleosides, bases and their analogues in natural and cultured Cordyceps by pressurized liquid extraction and high performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS)".Analytica Chimica Acta 567.2(2006):218.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Fan H.]'s Articles
[Li S.P.]'s Articles
[Xiang J.J.]'s Articles
Baidu academic
Similar articles in Baidu academic
[Fan H.]'s Articles
[Li S.P.]'s Articles
[Xiang J.J.]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Fan H.]'s Articles
[Li S.P.]'s Articles
[Xiang J.J.]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.