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A rapid method for simultaneous determination of 15 flavonoids in Epimedium using pressurized liquid extraction and ultra-performance liquid chromatography
Chen X.J.1,2; Ji H.3; Zhang Q.W.1; Tu P.F.2; Wang Y.T.1; Guo B.L.4; Li S.P.1
2008
Source PublicationJournal of Pharmaceutical and Biomedical Analysis
ISSN7317085
Volume46Issue:2Pages:226
Abstract

Herba Epimedii (family Berberidaceae), Yinyanghuo in Chinese, is one of commonly used Chinese medicines. Flavonoids are considered as its active components. In this study, a rapid ultra-performance liquid chromatography (UPLC) method was developed for simultaneous determination of 15 flavonoids, including hexandraside E, kaempferol-3-O-rhamnoside, hexandraside F, epimedin A, epimedin B, epimedin C, icariin, epimedoside C, baohuoside II, caohuoside C, baohuoside VII, sagittatoside A, sagittatoside B, 2″-O-rhamnosyl icariside II and baohuoside I in different species of Epimedium. The analysis was performed on Waters Acquity UPLC system with an Acquity UPLC BEH C18 column (50 mm × 2.1 mm I.D., 1.7 μm) and gradient elution of 50 mM acetic acid aqueous solution and acetonitrile within 12 min. All calibration curves showed good linearity (R2 > 0.9997) within test ranges. The LOD and LOQ were lower than 0.13 and 0.52 ng on column, respectively. The R.S.D.s for intra- and inter-day of 15 analytes were less than 5.0% at three levels, and the recoveries were 95.0-103.7%. The validated method was successfully applied to quantitatively analyze 15 flavonoids in different species of Epimedium. The results showed there were great variations among the contents of investigated flavonoids. Hierarchical clustering analysis based on characteristics of 15 investigated compounds peaks in UPLC profiles showed that 37 samples were divided into 3 main clusters, which were in accordance with their flavonoids contents. The simulative mean chromatogram of the high content cluster was generated to compare the samples from different species and/or locations of Epimedium. Four flavonoids including epimedin A, B, C and icariin were selected as markers for quality control of the species of Epimedium used as Yinyanghuo. © 2007 Elsevier B.V. All rights reserved.

KeywordEpimedium Flavonoids Hierarchical Clustering Analysis Pressurized Liquid Extraction (Ple) Similarity Evaluation Ultra-performance Liquid Chromatography (Uplc)
DOIhttp://doi.org/10.1016/j.jpba.2007.09.016
URLView the original
Indexed BySCI
Language英语
WOS Research AreaChemistry ; Pharmacology & Pharmacy
WOS SubjectChemistry, Analytical ; Pharmacology & Pharmacy
WOS IDWOS:000253011400003
The Source to ArticleScopus
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Cited Times [WOS]:82   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorLi S.P.
Affiliation1.Institute of Chinese Medical Sciences, University of Macau, Taipa, Macao SAR, China
2.Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University, Beijing 100083, China
3.Department of Pharmacology, China Pharmaceutical University, Nanjing 210009, China
4.Institute of Medicinal Plants Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100094, China
First Author AffilicationInstitute of Chinese Medical Sciences
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Chen X.J.,Ji H.,Zhang Q.W.,et al. A rapid method for simultaneous determination of 15 flavonoids in Epimedium using pressurized liquid extraction and ultra-performance liquid chromatography[J]. Journal of Pharmaceutical and Biomedical Analysis,2008,46(2):226.
APA Chen X.J..,Ji H..,Zhang Q.W..,Tu P.F..,Wang Y.T..,...&Li S.P..(2008).A rapid method for simultaneous determination of 15 flavonoids in Epimedium using pressurized liquid extraction and ultra-performance liquid chromatography.Journal of Pharmaceutical and Biomedical Analysis,46(2),226.
MLA Chen X.J.,et al."A rapid method for simultaneous determination of 15 flavonoids in Epimedium using pressurized liquid extraction and ultra-performance liquid chromatography".Journal of Pharmaceutical and Biomedical Analysis 46.2(2008):226.
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