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Conjugating a groove-binding motif to an Ir(iii) complex for the enhancement of G-quadruplex probe behavior
Modi Wang1; Zhifeng Mao1; Tian-Shu Kang2; Chun-Yuen Wong3; Jean-Louis Mergny4,5; Chung-Hang Leung2; Dik-Lung Ma1
2016
Source PublicationChemical Science
ISSN20416520
Volume7Issue:4Pages:2516
Abstract

In this study, the reported G-quadruplex groove binder benzo[d,e]isoquinoline was linked to a cyclometallated Ir(iii) complex to generate a highly selective DNA probe 1 that retains the favorable photophysical properties of the parent complex. The linked complex 1 showed advantages of both parent complex 2 and groove binder 3. Similar to 3, the conjugated complex 1 exhibits a superior affinity and selectivity for G-quadruplex DNA over other conformations of DNA or proteins, with the fold enhancement ratio obviously improved compared with parent complex 2. The molecular modelling revealed a groove-binding mode between complex 1 and G-quadruplex. Meanwhile 1 also possesses the prominent advantages of transition metal complex probes such as a large Stokes shift and long lifetime phosphorescence, which could be recognized in strong fluorescence media through time-resolved emission spectroscopy (TRES). We then employed 1 to develop a detection assay for AGR2, a potential cancer biomarker, as a "proof-of-principle" demonstration of the application of a linked complex for DNA-based detection in diluted fetal bovine serum. We anticipate that this conjugation method may be further employed in the development of DNA probes and have applications in label-free DNA-based diagnostic platforms.

DOI10.1039/c6sc00001k
URLView the original
Indexed BySCIE ; IC
Language英语
WOS Research AreaChemistry
WOS SubjectChemistry, Multidisciplinary
WOS IDWOS:000372614800006
The Source to ArticleScopus
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Cited Times [WOS]:131   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorJean-Louis Mergny; Chung-Hang Leung; Dik-Lung Ma
Affiliation1.Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China
2.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China
3.Department of Biology and Chemistry, City University of Hong Kong, Kowloon Tong, Hong Kong, China
4.University of Bordeaux, ARNA Laboratory, Bordeaux, France
5.INSERM, U869, IECB, Pessac, France
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Modi Wang,Zhifeng Mao,Tian-Shu Kang,et al. Conjugating a groove-binding motif to an Ir(iii) complex for the enhancement of G-quadruplex probe behavior[J]. Chemical Science,2016,7(4):2516.
APA Modi Wang.,Zhifeng Mao.,Tian-Shu Kang.,Chun-Yuen Wong.,Jean-Louis Mergny.,...&Dik-Lung Ma.(2016).Conjugating a groove-binding motif to an Ir(iii) complex for the enhancement of G-quadruplex probe behavior.Chemical Science,7(4),2516.
MLA Modi Wang,et al."Conjugating a groove-binding motif to an Ir(iii) complex for the enhancement of G-quadruplex probe behavior".Chemical Science 7.4(2016):2516.
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