UM
Conjugating a groove-binding motif to an Ir(iii) complex for the enhancement of G-quadruplex probe behavior
Wang M.; Mao Z.; Kang T.-S.; Wong C.-Y.; Mergny J.-L.; Leung C.-H.; Ma D.-L.
2016
Source PublicationChemical Science
ISSN20416520
Volume7Issue:4Pages:2516
AbstractIn this study, the reported G-quadruplex groove binder benzo[d,e]isoquinoline was linked to a cyclometallated Ir(iii) complex to generate a highly selective DNA probe 1 that retains the favorable photophysical properties of the parent complex. The linked complex 1 showed advantages of both parent complex 2 and groove binder 3. Similar to 3, the conjugated complex 1 exhibits a superior affinity and selectivity for G-quadruplex DNA over other conformations of DNA or proteins, with the fold enhancement ratio obviously improved compared with parent complex 2. The molecular modelling revealed a groove-binding mode between complex 1 and G-quadruplex. Meanwhile 1 also possesses the prominent advantages of transition metal complex probes such as a large Stokes shift and long lifetime phosphorescence, which could be recognized in strong fluorescence media through time-resolved emission spectroscopy (TRES). We then employed 1 to develop a detection assay for AGR2, a potential cancer biomarker, as a "proof-of-principle" demonstration of the application of a linked complex for DNA-based detection in diluted fetal bovine serum. We anticipate that this conjugation method may be further employed in the development of DNA probes and have applications in label-free DNA-based diagnostic platforms. © The Royal Society of Chemistry 2016.
DOI10.1039/c6sc00001k
URLView the original
Language英语
The Source to ArticleScopus
Fulltext Access
Citation statistics
Cited Times [WOS]:123   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Recommended Citation
GB/T 7714
Wang M.,Mao Z.,Kang T.-S.,et al. Conjugating a groove-binding motif to an Ir(iii) complex for the enhancement of G-quadruplex probe behavior[J]. Chemical Science,2016,7(4):2516.
APA Wang M..,Mao Z..,Kang T.-S..,Wong C.-Y..,Mergny J.-L..,...&Ma D.-L..(2016).Conjugating a groove-binding motif to an Ir(iii) complex for the enhancement of G-quadruplex probe behavior.Chemical Science,7(4),2516.
MLA Wang M.,et al."Conjugating a groove-binding motif to an Ir(iii) complex for the enhancement of G-quadruplex probe behavior".Chemical Science 7.4(2016):2516.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wang M.]'s Articles
[Mao Z.]'s Articles
[Kang T.-S.]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wang M.]'s Articles
[Mao Z.]'s Articles
[Kang T.-S.]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wang M.]'s Articles
[Mao Z.]'s Articles
[Kang T.-S.]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.