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A rapid method for the simultaneous determination of 11 saponins in Panax notoginseng using ultra performance liquid chromatography
Guan J.; Lai C.M.; Li S.P.
2007
Source PublicationJournal of Pharmaceutical and Biomedical Analysis
ISSN7317085
Volume44Issue:4Pages:996
AbstractA rapid ultra performance liquid chromatography coupled with photo diode array detection method (UPLC-PDA) was developed for the simultaneous determination of 11 saponins, namely notoginsenoside R1, ginsenoside Rg1, Re, Rf, Rb1, Rg2, Rc, Rb2, Rb3, Rd and Rg3 in Panax notoginseng. The analysis was performed on Acquity UPLC system with Acquity UPLC BEH C18 column and gradient elution of water and acetonitrile in 12 min. The high correlation coefficient (r2 > 0.9968) values indicated good correlations between the investigated compounds' concentrations and their peak areas within the test ranges. The LOQ and LOD were lower to 0.2-2.4 and 0.1-1.8 ng on column, respectively. The overall intra- and inter-day variations (R.S.D.) of 11 saponins were lower than 3.1%. The developed method was successfully used for the analysis of saponins in P. notoginseng with overall recovery of 93.0-101.6% for the analytes. The results show that UPLC is a powerful tool for analysis of components in Chinese medicines. © 2007 Elsevier B.V. All rights reserved.
KeywordGinsenoside Notoginsenoside Panax notoginseng Pressurized liquid extraction Ultra performance liquid chromatography
DOI10.1016/j.jpba.2007.03.032
URLView the original
Language英语
The Source to ArticleScopus
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Cited Times [WOS]:96   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
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GB/T 7714
Guan J.,Lai C.M.,Li S.P.. A rapid method for the simultaneous determination of 11 saponins in Panax notoginseng using ultra performance liquid chromatography[J]. Journal of Pharmaceutical and Biomedical Analysis,2007,44(4):996.
APA Guan J.,Lai C.M.,&Li S.P..(2007).A rapid method for the simultaneous determination of 11 saponins in Panax notoginseng using ultra performance liquid chromatography.Journal of Pharmaceutical and Biomedical Analysis,44(4),996.
MLA Guan J.,et al."A rapid method for the simultaneous determination of 11 saponins in Panax notoginseng using ultra performance liquid chromatography".Journal of Pharmaceutical and Biomedical Analysis 44.4(2007):996.
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