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Label-free luminescence switch-on detection of hepatitis C virus NS3 helicase activity using a G-quadruplex-selective probe
Leung K.-H.1; He H.-Z.1; He B.1; Zhong H.-J.2; Lin S.1; Wang Y.-T.2; Ma D.-L.1; Leung C.-H.2
2015
Source PublicationChemical Science
ISSN20416520
Volume6Issue:4Pages:2166-2171
Abstract

A series of luminescent Ir(iii) complexes were synthesised and evaluated for their ability to act as luminescent G-quadruplex-selective probes. The Ir(iii) complex 9, [Ir(phq)2(phen)]PF6 (where phq = 2-phenylquinoline; phen = 1,10-phenanthroline), exhibited high luminescence in the presence of G-quadruplex DNA compared to dsDNA and ssDNA, and was employed to construct a label-free G-quadruplex-based assay for hepatitis C virus NS3 helicase activity in aqueous solution. Moreover, the application of the assay for screening potential helicase inhibitors was demonstrated. To our knowledge, this is the first G-quadruplex-based assay for helicase activity.

DOIhttp://doi.org/10.1039/c4sc03319a
URLView the original
Indexed BySCI
Language英语
WOS Research AreaChemistry
WOS SubjectChemistry, Multidisciplinary
WOS IDWOS:000351412800004
The Source to ArticleScopus
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Cited Times [WOS]:120   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorMa D.-L.; Leung C.-H.
Affiliation1.Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China
2.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Leung K.-H.,He H.-Z.,He B.,et al. Label-free luminescence switch-on detection of hepatitis C virus NS3 helicase activity using a G-quadruplex-selective probe[J]. Chemical Science,2015,6(4):2166-2171.
APA Leung K.-H..,He H.-Z..,He B..,Zhong H.-J..,Lin S..,...&Leung C.-H..(2015).Label-free luminescence switch-on detection of hepatitis C virus NS3 helicase activity using a G-quadruplex-selective probe.Chemical Science,6(4),2166-2171.
MLA Leung K.-H.,et al."Label-free luminescence switch-on detection of hepatitis C virus NS3 helicase activity using a G-quadruplex-selective probe".Chemical Science 6.4(2015):2166-2171.
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