UM  > 中華醫藥研究院
Detection of nicking endonuclease activity using a G-quadruplex-selective luminescent switch-on probe
Lu L.1; Shiu-Hin Chan D.1; Kwong D.W.J.1; He H.-Z.1; Leung C.-H.2; Ma D.-L.1
2014
Source PublicationChemical Science
ISSN20416520
Volume5Issue:12Pages:4561
Abstract

A series of luminescent Ir(iii) complexes were synthesised and evaluated for their ability to act as G-quadruplex-selective probes. A novel Ir(iii) complex was found to be highly selective for G-quadruplex DNA, and was employed in a label-free G-quadruplex-based detection assay for nicking endonuclease activity in aqueous solution. A proof-of-concept of this probe has been demonstrated by using Nb·BbvCI as a model enzyme. In this assay, a DNA substrate comprised of oligonucleotides ON1 (5′-GTG3TAG3CG3T2G2CTGAG2TGA-3′) and ON2 (5′-TCAC2TCAGC2A2C2-3′) initially exists in a duplex conformation, resulting in a low luminescence signal due to the weak interaction between the Ir(iii) complex and duplex DNA. Upon cleavage by Nb·BbvCI, the guanine-rich sequence is released and folds into a G-quadruplex, which greatly enhances the luminescence of the Ir(iii) probe. This method was highly sensitive for Nb·BbvCI over other DNA-modifying enzymes. This journal is © the Partner Organisations 2014.

DOI10.1039/c4sc02032d
URLView the original
Indexed BySCI
Language英语
WOS Research AreaChemistry
WOS SubjectChemistry, Multidisciplinary
WOS IDWOS:000344376400003
The Source to ArticleScopus
Fulltext Access
Citation statistics
Cited Times [WOS]:118   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorHe H.-Z.; Leung C.-H.; Ma D.-L.
Affiliation1.Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, China
2.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Lu L.,Shiu-Hin Chan D.,Kwong D.W.J.,et al. Detection of nicking endonuclease activity using a G-quadruplex-selective luminescent switch-on probe[J]. Chemical Science,2014,5(12):4561.
APA Lu L.,Shiu-Hin Chan D.,Kwong D.W.J.,He H.-Z.,Leung C.-H.,&Ma D.-L..(2014).Detection of nicking endonuclease activity using a G-quadruplex-selective luminescent switch-on probe.Chemical Science,5(12),4561.
MLA Lu L.,et al."Detection of nicking endonuclease activity using a G-quadruplex-selective luminescent switch-on probe".Chemical Science 5.12(2014):4561.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Lu L.]'s Articles
[Shiu-Hin Chan D.]'s Articles
[Kwong D.W.J.]'s Articles
Baidu academic
Similar articles in Baidu academic
[Lu L.]'s Articles
[Shiu-Hin Chan D.]'s Articles
[Kwong D.W.J.]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Lu L.]'s Articles
[Shiu-Hin Chan D.]'s Articles
[Kwong D.W.J.]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.