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Chemical characteristics for different parts of Panax notoginseng using pressurized liquid extraction and HPLC-ELSD
Wan J.B.1; Yang F.Q.1; Li S.P.1; Wang Y.T.1; Cui X.M.2
2006
Source PublicationJournal of Pharmaceutical and Biomedical Analysis
ISSN7317085
Volume41Issue:5Pages:1596
Abstract

The chemical characteristics for different parts of Panax notoginseng, including root, fibre root, rhizome, stem, leaf, flower and seed, were determined using high performance liquid chromatography-evaporative light scattering detection (HPLC-ELSD) and pressurized liquid extraction (PLE). Eight major saponins, namely notoginsenoside R1, ginsenosides Rg1, Re, Rb1, Rc, Rb2, Rb3 and Rd were also quantitatively compared among the different parts of P. notoginseng. The chromatograms showed that there was significant difference between underground (root, fibre root, rhizome) and aerial (leaf and flower) parts from P. notoginseng, though the similarities of entire chromatographic patterns among tested samples from underground (0.965 ± 0.029, n = 12) and aerial parts (0.987 ± 0.014, n = 5) were similar, respectively. Especially, no saponin was detected in the seed of P. notoginseng. Hierarchical clustering analysis based on eight investigated saponins or the ratios of contents for ginsenoside Rg1/Rb1 and ginsenoside Rb3/Rb1 showed that the samples from different parts of P. notoginseng were divided into three main clusters. One cluster was underground parts, which contained rich protopanaxatriol and protopanaxadiol types saponins. The leaf and flower were in the same cluster, which contained protopanaxadiol type saponins only. Especially, ginsenoside Rc, Rb2 and Rb3, rare in the underground parts, were rich in aerial parts of P. notoginseng. The stem of P. notoginseng was another cluster. Based on the cluster analysis, the chemical characteristics for different parts of P. notoginseng were revealed. They are composite cluster (underground parts), protopanaxadiol cluster (aerial parts) and interim (stem) cluster, which was the one between the two typical clusters, respectively. The result shows that chemical characteristics of underground parts and aerial parts from P. notoginseng are obviously different, which is helpful for pharmacological evaluation and quality control of P. notoginseng. © 2006 Elsevier B.V. All rights reserved.

KeywordGinsenoside High Performance Liquid Chromatography-evaporative Light Scattering Detection Notoginsenoside Panax Notoginseng Pressurized Liquid Extraction
DOIhttp://doi.org/10.1016/j.jpba.2006.01.058
URLView the original
Indexed BySCI
Language英语
WOS Research AreaChemistry ; Pharmacology & Pharmacy
WOS SubjectChemistry, Analytical ; Pharmacology & Pharmacy
WOS IDWOS:000241343800013
The Source to ArticleScopus
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Cited Times [WOS]:109   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorLi S.P.
Affiliation1.Institute of Chinese Medical Sciences, University of Macau, Taipa, Macau, China
2.Wenshan Prefecture Sanqi Research Institute, Wenshan, Yunnan, China
First Author AffilicationInstitute of Chinese Medical Sciences
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Wan J.B.,Yang F.Q.,Li S.P.,et al. Chemical characteristics for different parts of Panax notoginseng using pressurized liquid extraction and HPLC-ELSD[J]. Journal of Pharmaceutical and Biomedical Analysis,2006,41(5):1596.
APA Wan J.B.,Yang F.Q.,Li S.P.,Wang Y.T.,&Cui X.M..(2006).Chemical characteristics for different parts of Panax notoginseng using pressurized liquid extraction and HPLC-ELSD.Journal of Pharmaceutical and Biomedical Analysis,41(5),1596.
MLA Wan J.B.,et al."Chemical characteristics for different parts of Panax notoginseng using pressurized liquid extraction and HPLC-ELSD".Journal of Pharmaceutical and Biomedical Analysis 41.5(2006):1596.
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