Conditional deletion of FOXL2 and SMAD4 in gonadotropes of adult mice causes isolated FSH deficiency
Li,Yining1; Schang,Gauthier1; Wang,Ying1; Zhou,Xiang1; Levasseur,Adrien2; Boyer,Alexandre2; Deng,Chu Xia3; Treier,Mathias4,5; Boehm,Ulrich6; Boerboom,Derek2; Bernard,Daniel J.1
Source PublicationEndocrinology
AbstractThe glycoprotein FSH, a product of pituitary gonadotrope cells, regulates ovarian follicle development in females and spermatogenesis in males. FSH is a heterodimer of the common a gonadotropin subunit and the hormone-specific FSHb subunit (a product of the Fshb gene). Using a conditional knockout approach (Cre-lox), we previously demonstrated that Fshb expression in mice depends on the transcription factors forkhead box L2 (FOXL2) and SMAD4. Deletion of Foxl2 or Smad4 alone led to FSH deficiency, female subfertility, and oligozoospermia in males. Simultaneous deletion of the two genes yielded a greater suppression of FSH and female sterility. The Cre-driver used previously was first active during embryonic development. Therefore, it is unclear whether FOXL2 and SMAD4 play important roles in the development or adult function of gonadotropes, or both. To address this question, we developed a tamoxifen-inducible Cre-driver line, which enabled Foxl2 and Smad4 gene deletions in gonadotropes of adult mice. After tamoxifen treatment, females with previously demonstrated fertility exhibited profound reductions in FSH levels, arrested ovarian follicle development, and sterility. FSH levels were comparably reduced in males 1 or 2 months after treatment; however, spermatogenesis was unaffected. These data indicate that (1) FOXL2 and SMAD4 are necessary to maintain FSH synthesis in gonadotrope cells of adult mice, (2) FSH is essential for female reproduction but appears to be unnecessary for the maintenance of spermatogenesis in adult male mice, and (3) the inducible Cre-driver line developed here provides a powerful tool to interrogate gene function in gonadotrope cells of adult mice.
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Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.Department of Pharmacology and Therapeutics,McGill University,Montreál,H3G1Y6,Canada
2.Département de Biomé Decine Vé Té Rinaire,Université de Montré Al,St-Hyacinthe,J2S7C6,Canada
3.Faculty of Health Sciences,University of Macau,999078,Macao
4.Cardiovascular and Metabolic Sciences,Max Delbrü Ck Center for Molecular Medicine in the Helmholtz Association,Berlin-Buch,13125,Germany
5.Charité-Universitä Tsmedizin Berlin,Berlin,10117,Germany
6.Department of Experimental Pharmacology,Center for Molecular Signaling,Saarland University,School of Medicine,Homburg,66421,Germany
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GB/T 7714
Li,Yining,Schang,Gauthier,Wang,Ying,et al. Conditional deletion of FOXL2 and SMAD4 in gonadotropes of adult mice causes isolated FSH deficiency[J]. Endocrinology,2018,159(7):2641-2655.
APA Li,Yining.,Schang,Gauthier.,Wang,Ying.,Zhou,Xiang.,Levasseur,Adrien.,...&Bernard,Daniel J..(2018).Conditional deletion of FOXL2 and SMAD4 in gonadotropes of adult mice causes isolated FSH deficiency.Endocrinology,159(7),2641-2655.
MLA Li,Yining,et al."Conditional deletion of FOXL2 and SMAD4 in gonadotropes of adult mice causes isolated FSH deficiency".Endocrinology 159.7(2018):2641-2655.
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