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Deficiency in Fpr2 results in reduced numbers of Lin(-)cKit(+)Sca1(+) myeloid progenitor cells
Chen, Keqiang; Singh, Vijay K.; Tang, Peng; Bao, Zhiyao; He, Tianzhen; Xiang, Yi; Gong, Wanghua; Yoshimura, Teizo; Le, Yingying; Tessarollo, Lino; Chen, Xin; Wang, Ji Ming
2018-08-31
Source PublicationJOURNAL OF BIOLOGICAL CHEMISTRY
ISSN0021-9258
Volume293Issue:35Pages:13452-13463
AbstractThe Lin(-)c-Kit(+) Sca-1(+) cell population in the bone marrow (BM) serves as the direct precursor for differentiation of myeloid cells. In this study, we report that deficiency in Fpr2, a G protein-coupled chemoattractant receptor in mice, is associated with reduced BM nucleated cells, including CD31(+)Ly6C(+) (granulocytes and monocytes), CD31(-)/Ly6C(int) (granuloid cells), and CD31(-)/Ly6C(high) (predominantly monocytes) cells. In particular, the number of Lin(-)c-Kit(+)Sca-1(+) (LKS) cells was reduced in Fpr2(-/-) mouse BM. This was supported by observations of the reduced incorporation of intraperitoneally injected bromodeoxyuridine by cells in the c-Kit(+) population from Fpr2(-/-) mouse BM. Purified c-Kit(+) cells from Fpr2(-/-) mice showed reduced expansion when cultured in vitro with stem cell factor (SCF). SCF/c-Kit-mediated phosphorylation of P38, STAT1, Akt (Thr-308), and Akt (Ser-473) was also significantly reduced in c-Kit(+) cells from Fpr2(-/-) mice. Furthermore, Fpr2 agonists enhanced SCF-induced proliferation of c-Kit(+) cells. Colony-forming unit assays revealed that CFU-granulocyte-macrophage formation of BM cells from Fpr2(-/-) mice was significantly reduced. After heat-inactivated bacterial stimulation in the airway, the expansion of c-kit(+) Sca-1(+) cells in BM and recruitment of Ly6G(+) cells to the lungs and CD11b(+)Ly6C(+)TNF(+) cells to the spleen of Fpr2(-/-) mice was significantly reduced. These results demonstrate an important role for Fpr2 in the development of myeloid lineage precursors in mouse BM.
Keywordmouse bone marrow myeloid cell proliferation cytokine c-KIT Fpr2 Lin(-)c-Kit(+)Sca1(+)cells SCF
DOI10.1074/jbc.RA118.002683
URLView the original
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemistry & Molecular Biology
WOS IDWOS:000443375500009
PublisherAMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
The Source to ArticleWOS
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Cited Times [WOS]:2   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Recommended Citation
GB/T 7714
Chen, Keqiang,Singh, Vijay K.,Tang, Peng,et al. Deficiency in Fpr2 results in reduced numbers of Lin(-)cKit(+)Sca1(+) myeloid progenitor cells[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2018,293(35):13452-13463.
APA Chen, Keqiang.,Singh, Vijay K..,Tang, Peng.,Bao, Zhiyao.,He, Tianzhen.,...&Wang, Ji Ming.(2018).Deficiency in Fpr2 results in reduced numbers of Lin(-)cKit(+)Sca1(+) myeloid progenitor cells.JOURNAL OF BIOLOGICAL CHEMISTRY,293(35),13452-13463.
MLA Chen, Keqiang,et al."Deficiency in Fpr2 results in reduced numbers of Lin(-)cKit(+)Sca1(+) myeloid progenitor cells".JOURNAL OF BIOLOGICAL CHEMISTRY 293.35(2018):13452-13463.
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