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Magneto-controlled flow-injection device for electrochemical immunoassay of alpha-fetoprotein on magnetic beads using redox-active ferrocene derivative polymer nanospheres
Bin Li1,2; Wenyuan Pu1; Houxi Xu1; Lilin Ge1; Hang Fai Kwok2; Lihong Hu1
2019-02
Source PublicationANALYST
ISSN0003-2654
Volume144Issue:4Pages:1433-1441
AbstractA new electrochemical immunosensing protocol by coupling with a magneto-controlled flow-through microfluidic device was developed for the sensitive detection of alpha-fetoprotein (AFP) on magnetic beads (MB) using ferrocene derivative polymer nanospheres (FDNP) as the electroactive mediators. The immunosensing probe was prepared by covalent conjugation of monoclonal mouse anti-human AFP antibodies with magnetic beads, while the recognition element was constructed by means of immobilizing polyclonal rabbit anti-human AFP antibodies on the redox FDNP. Upon target AFP introduction, the sandwich-type immunoreaction was carried out between the immunosensing probe and the recognition element, and the formed immunocomplex was captured in the detection cell with an external magnet. Ferrocene polymer nanospheres synthesized by infinite coordination polymerization were utilized as the signal-generation tags during the electrochemical measurement. Under optimal conditions, the magneto-controlled flow-through immunosensing platform exhibited good electrochemical responses toward target AFP within a dynamic working range of 0.01–100 ng mL−1 and with a low detection limit of 5.7 pg mL−1. The nanoparticles-based sensing systems also gave good reproducibility, high specificity and long-term stability. Moreover, our strategy displayed well-matched accuracy for the analysis of human serum specimens relative to commercial Roche 2010 Electrochemiluminescence (ECL) Automated Analyzer.
DOI10.1039/c8an01978a
Indexed BySCI
Language英语
WOS Research AreaChemistry
WOS SubjectChemistry, Analytical
WOS IDWOS:000459637300039
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Cited Times [WOS]:2   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Affiliation1.Jiangsu Key Laboratory for Functional Substance of Chinese Medicine, Nanjing University of Chinese Medicine, 138 Xianlin Avenue, Qixia district, Nanjing, China
2.Institute of Translational Medicine, Faculty of Health Sciences, University of Macau, Avenida de Universidade, Macau SAR
First Author AffilicationFaculty of Health Sciences
Recommended Citation
GB/T 7714
Bin Li,Wenyuan Pu,Houxi Xu,et al. Magneto-controlled flow-injection device for electrochemical immunoassay of alpha-fetoprotein on magnetic beads using redox-active ferrocene derivative polymer nanospheres[J]. ANALYST,2019,144(4):1433-1441.
APA Bin Li,Wenyuan Pu,Houxi Xu,Lilin Ge,Hang Fai Kwok,&Lihong Hu.(2019).Magneto-controlled flow-injection device for electrochemical immunoassay of alpha-fetoprotein on magnetic beads using redox-active ferrocene derivative polymer nanospheres.ANALYST,144(4),1433-1441.
MLA Bin Li,et al."Magneto-controlled flow-injection device for electrochemical immunoassay of alpha-fetoprotein on magnetic beads using redox-active ferrocene derivative polymer nanospheres".ANALYST 144.4(2019):1433-1441.
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