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Rapid discrimination of colon cancer cells with single base mutation in KRAS gene segment using laser tweezers Raman spectroscopy
Mengmeng Liu1; Xiujie Liu1; Zufang Huang1; Xiaoqiong Tang2; Xueliang Lin1; Yunchao Xu1; Guannan Chen1; Hang Fai Kwok3; Yao Lin2; Shangyuan Feng1
2019-03
Source PublicationJOURNAL OF BIOPHOTONICS
ISSN1864-063X
Volume12Issue:3
AbstractAbstract Laser tweezers Raman spectroscopy (LTRS) as a label‐free and noninvasive technology has been proven to be an ideal tool for analysis of single living cells, which provides important fingerprint information without interference from surrounding environments. For the first time, LTRS system was successfully used to examine the colon cancer cells with single base mutation in KRAS gene segment, including DKS‐8 (KRAS wild‐type [WT]) and DLD‐1 (KRAS mutant‐type [MT]), HKE‐3 (KRAS WT) and HCT‐116 (KRAS MT). Spectra changes of some vital biomolecules due to the gene mutation state were sensitively recorded by our home‐made LTRS system. As a result of the comparison between DKS‐8 and DLD‐1 cells, an index of 97.5% of correct classification was obtained by combining LTRS with principle component analysis coupled with linear discriminant analysis (PCA‐LDA) statistical analysis, while an index of 97.0% of correct classification was achieved between HKE‐3 and HCT‐116 cells. Moreover, between WT cells (DKS‐8 and HKE‐3) vs MT cells (DLD‐1 and HCT‐116), the index of correct classification was 81.2%, which was quite encouraging. Our preliminary results showed that the LTRS system coupled with PCA‐LDA analysis will have a great potential for further applications in the rapid and label‐free detection of circulating tumor cells in liquid biopsy.
KeywordCancer Cell Screening Kras Mutant Optical Tweezers Raman Spectroscopy Single Base Mutation
DOI10.1002/jbio.201800332
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Biophysics ; Optics
WOS SubjectBiochemical Research Methods ; Biophysics ; Optics
WOS IDWOS:000462683300019
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Cited Times [WOS]:3   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Affiliation1.Fujian Normal University, Ministry of Education, Key Laboratory of Optoelectronic Science and Technology for Medicine, Fujian Provincial Key Laboratory for Photonics Technology, Fuzhou, China
2.Fujian Normal University, College of Life Sciences, Fuzhou, China
3.Faculty of Health Sciences, Cancer Centre, University of Macau, Avenida de Universidade, Taipa, China
Recommended Citation
GB/T 7714
Mengmeng Liu,Xiujie Liu,Zufang Huang,et al. Rapid discrimination of colon cancer cells with single base mutation in KRAS gene segment using laser tweezers Raman spectroscopy[J]. JOURNAL OF BIOPHOTONICS,2019,12(3).
APA Mengmeng Liu.,Xiujie Liu.,Zufang Huang.,Xiaoqiong Tang.,Xueliang Lin.,...&Shangyuan Feng.(2019).Rapid discrimination of colon cancer cells with single base mutation in KRAS gene segment using laser tweezers Raman spectroscopy.JOURNAL OF BIOPHOTONICS,12(3).
MLA Mengmeng Liu,et al."Rapid discrimination of colon cancer cells with single base mutation in KRAS gene segment using laser tweezers Raman spectroscopy".JOURNAL OF BIOPHOTONICS 12.3(2019).
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