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Continuous artificial synthesis of glucose precursor using enzyme-immobilized microfluidic reactors
Yujiao Zhu1,2; Ziyu Huang2; Qingming Chen1; Qian Wu4,5; Xiaowen Huang3; Pui-Kin So6; Liyang Shao7; Zhongping Yao4,5; Yanwei Jia2,8,9; Zhaohui Li10,11; Weixing Yu12; Yi Yang13; Aoqun Jian14; Shengbo Sang14; Wendong Zhang14; Xuming Zhang1
2019-09
Source PublicationNATURE COMMUNICATIONS
ISSN2041-1723
Volume10
AbstractFood production in green crops is severely limited by low activity and poor specificity of D-ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) in natural photosynthesis (NPS). This work presents a scientific solution to overcome this problem by immobilizing RuBisCO into a microfluidic reactor, which demonstrates a continuous production of glucose precursor at 13.8 μmol g−1 RuBisCO min−1 from CO2 and ribulose-1,5-bisphosphate. Experiments show that the RuBisCO immobilization significantly enhances enzyme stabilities (7.2 folds in storage stability, 6.7 folds in thermal stability), and also improves the reusability (90.4% activity retained after 5 cycles of reuse and 78.5% after 10 cycles). This work mimics the NPS pathway with scalable microreactors for continuous synthesis of glucose precursor using very small amount of RuBisCO. Although still far from industrial production, this work demonstrates artificial synthesis of basic food materials by replicating the light-independent reactions of NPS, which may hold the key to food crisis relief and future space colonization.
DOI10.1038/s41467-019-12089-6
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000484599200001
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Citation statistics
Cited Times [WOS]:3   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
INSTITUTE OF MICROELECTRONICS
Affiliation1.Department of Applied Physics, The Hong Kong Polytechnic University, Hong Kong, China
2.State Key Laboratory of Analog and Mixed Signal VLSI, Institute of Microelectronics, University of Macau, Macau, China
3.State Key Laboratory of Biobased Material and Green Papermaking; Shandong Provincial Key Laboratory of Microbial Engineering, Department of Bioengineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, China
4.State Key Laboratory for Chemical Biology and Drug Discovery, Food Safety and Technology Research Centre, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hong Kong, China
5.State Key Laboratory of Chinese Medicine and Molecular Pharmacology (Incubation), Shenzhen Key Laboratory of Food Biological Safety Control, Shenzhen Research Institute of the Hong Kong Polytechnic University, Shenzhen, China
6.University of Research Facility in Life Sciences, The Hong Kong Polytechnic University, Hong Kong, China
7.Department of Electrical and Electronic Engineering, Southern University of Science and Technology, Shenzhen, China
8.Faculty of Science and Technology, University of Macau, Macau, China
9.Faculty of Health Sciences, University of Macau, Macau, China
10.School of Electronics and Information Engineering, Sun Yat-Sen University, Guangzhou, China
11.Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai, China
12.Key Laboratory of Spectral Imaging Technology, Xi’an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi’an, China
13.School of Physics & Technology, Wuhan University, Wuhan, China
14.MircoNano System Research Center, College of Information and Computer Science, Taiyuan University of Technology, Taiyuan, China
First Author AffilicationUniversity of Macau
Recommended Citation
GB/T 7714
Yujiao Zhu,Ziyu Huang,Qingming Chen,et al. Continuous artificial synthesis of glucose precursor using enzyme-immobilized microfluidic reactors[J]. NATURE COMMUNICATIONS,2019,10.
APA Yujiao Zhu.,Ziyu Huang.,Qingming Chen.,Qian Wu.,Xiaowen Huang.,...&Xuming Zhang.(2019).Continuous artificial synthesis of glucose precursor using enzyme-immobilized microfluidic reactors.NATURE COMMUNICATIONS,10.
MLA Yujiao Zhu,et al."Continuous artificial synthesis of glucose precursor using enzyme-immobilized microfluidic reactors".NATURE COMMUNICATIONS 10(2019).
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