UM  > 中華醫藥研究院
METHOD OF DETECTING HELICASE ACTIVITY
Dik Lung Ma; Chung Hang Leung; Hai Jing Zhong; Bingyong He
Rights HolderUNIVERSITY OF MACAU ; HONG KONG BAPTIST UNIVERSITY
Date Available2017-05-25
CountryUnited States
Subtype发明专利
Abstract

The present invention provides a method of detecting helicase activity in a test solution, providing a substrate solution containing a double-stranded DNA, wherein said double-stranded DNA is a substrate for a helicase to be detected; (b) introducing the substrate solution to the test solution to form a reaction solution; (c) applying a luminescent probe into the reaction solution to form a mixture; and (d) measuring a luminescent response of the luminescent probe in the mixture, wherein the luminescent response corresponds to the helicase activity in the test solution. The present invention also refers to a method of screening a potent luminescent probe for detecting helicase activity, and a series of luminescent Ir (III) complexes. Said luminescent Ir (III) complex comprises an iridium-containing dimer and a nitrogen-containing ligand

Application Date2015-11-24
Patent NumberUS20170145472A1
Language英语
Application NumberUS14950538
Open (Notice) NumberUS20170145472A1
Fulltext Access
Document TypePatent
CollectionInstitute of Chinese Medical Sciences
AffiliationUniversity of Macau
Recommended Citation
GB/T 7714
Dik Lung Ma,Chung Hang Leung,Hai Jing Zhong,et al. METHOD OF DETECTING HELICASE ACTIVITY. US20170145472A1.
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