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L-Tetrahydropalamatine Inhibits Tumor Necrosis Factor-α-Induced Monocyte-Endothelial Cell Adhesion through Downregulation of Intercellular Adhesion Molecule-1 and Vascular Cell Adhesion Molecule-1 Involving Suppression of Nuclear Factor-κB Signaling Pathway
YANG Bin-rui1; YU Nan1; DENG Yan-hui1; HOI Pui Man1; YANG Bin2; LIU Guang-yu2; CONG Wei-hong2; Simon Ming-Yuen Lee1
2015-05
Source PublicationCHINESE JOURNAL OF INTEGRATIVE MEDICINE
ISSN1672-0415
Volume21Issue:5Pages:361-368
Abstract

OBJECTIVE:

To investigate whether I-tetrahydropalmatine (I-THP), an alkaloid mainly present in Corydalis family, could ameliorate early vascular inflammatory responses in atherosclerotic processes.

METHODS:

Fluorescently labeled monocytes were co-incubated with human umbilical vein endothelial cells (HUVECs), which were pretreated with I-THP and then simulated with tumor necrosis factor (TNF)-α in absence of I-THP to determine if I-THP could reduce thecytokine-induced adhesion of monocytes to HUVECs. Then I-THP were further studied the underlying mechanisms through observing the transcriptional and translational level of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) and the nuclear translocation of nuclear factor (NF)-κ B in HUVECs.

RESULTS:

L-THP could block TNF-α-induced adhesion of monocytes to HUVECs and could significantly inhibited the expression of ICAM-1 and VCAM-1 on cell surface by 31% and 36% at 30 μ mol/L. L-THP pretreatment could also markedly reduce transcriptional and translational level of VCAM-1 as well as mildly reduce the total protein and mRNA expression levels of ICAM-1. Furthermore, I-THP attenuated TNF-α-stimulated NF-κ B nuclear translocation.

CONCLUSION:

These results provide evidences supporting that I-THP could be a promising compound in the prevention and treatment of the early vascular inflammatory reaction in atherosclerosis by inhibiting monocyte adhesion to vascular endothelial cell through downregulating ICAM-1 and VCAM-1 in vascular endothelial cell based on suppressing NF-κ B.

KeywordL-tetrahydropalmatine Atherosclerosis Cell Adhesion Molecules Tumor Necrosis Factor-α Nuclear Factor-κb
DOI10.1007/s11655-015-2165-7
Indexed BySCI
Language英语
WOS Research AreaIntegrative & Complementary Medicine
WOS SubjectIntegrative & Complementary Medicine
WOS IDWOS:000353785800006
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Cited Times [WOS]:3   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China
2.Laboratory of Cardiovascular Diseases, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing (100091), China
Recommended Citation
GB/T 7714
YANG Bin-rui,YU Nan,DENG Yan-hui,et al. L-Tetrahydropalamatine Inhibits Tumor Necrosis Factor-α-Induced Monocyte-Endothelial Cell Adhesion through Downregulation of Intercellular Adhesion Molecule-1 and Vascular Cell Adhesion Molecule-1 Involving Suppression of Nuclear Factor-κB Signaling Pathway[J]. CHINESE JOURNAL OF INTEGRATIVE MEDICINE,2015,21(5):361-368.
APA YANG Bin-rui.,YU Nan.,DENG Yan-hui.,HOI Pui Man.,YANG Bin.,...&Simon Ming-Yuen Lee.(2015).L-Tetrahydropalamatine Inhibits Tumor Necrosis Factor-α-Induced Monocyte-Endothelial Cell Adhesion through Downregulation of Intercellular Adhesion Molecule-1 and Vascular Cell Adhesion Molecule-1 Involving Suppression of Nuclear Factor-κB Signaling Pathway.CHINESE JOURNAL OF INTEGRATIVE MEDICINE,21(5),361-368.
MLA YANG Bin-rui,et al."L-Tetrahydropalamatine Inhibits Tumor Necrosis Factor-α-Induced Monocyte-Endothelial Cell Adhesion through Downregulation of Intercellular Adhesion Molecule-1 and Vascular Cell Adhesion Molecule-1 Involving Suppression of Nuclear Factor-κB Signaling Pathway".CHINESE JOURNAL OF INTEGRATIVE MEDICINE 21.5(2015):361-368.
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