UM  > 中華醫藥研究院
Chinese herb pair Paeoniae Radix Alba and Atractylodis Macrocephalae Rhizoma suppresses LPS-induced inflammatory response through inhibiting MAPK and NF-κB pathway
Yangyang Zhou1; Hongxun Tao1; Anqi Wang1; Zhangfeng Zhong1; Xu Wu2; Mei Wang3; Zhaoxiang Bian4; Shengpeng Wang1,3; Yitao Wang1
2019-01
Source PublicationCHINESE MEDICINE
ISSN1749-8546
Volume14
Abstract

BackgroundThe combination of Radix Paeoniae Alba (RPA) and Rhizoma Atractylodis Macrocephalae (RAM) has long been used as a classic herb pair for the treatment of gynecologic and gastrointestinal diseases, but the underlying mechanisms of the herb pair remain unknown. This study aims to explore the anti-inflammatory potentials of RPA-RAM herb pair and to elucidate the underlying mechanisms.MethodsThe bioactive parts of RPA-RAM were extracted and screened through the inhibitory effects against nitric oxide (NO) production. The effects of optimized RPA-RAM extracts (OPAE) on inflammation-associated mediators were investigated by Western blotting, real-time quantitative PCR (RT-qPCR), Enzyme-linked immunosorbent (ELISA) and immunofluorescence staining.ResultsOPAE potently suppressed the productions of NO, TNF-, IL-6 and MCP-1 in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages, concentration-dependently inhibited protein level of inducible nitric oxide synthase (iNOS), dramatically downregulated mRNA expression of iNOS, TNF-, IL-6 and MCP-1. In addition, OPAE significantly prevented phosphorylation and degradation of inhibitory kappa B (IB) and subsequently restrained the nuclear translocation of NF-B p65. Pretreatment with OPAE also attenuated the LPS-induced phosphorylation of ERK, JNK and p38.ConclusionsOur findings demonstrated that OPAE suppressed inflammatory responses in LPS-stimulated RAW 264.7 macrophages by decreasing critical molecules involved in MAPK and NF-B pathway, suggesting that the herb pair could be a promising therapeutic candidate for inflammation-related diseases.

KeywordRadix Paeoniae Alba Rhizoma Atractylodis Macrocephalae Herb Pair Inflammatory
DOI10.1186/s13020-019-0224-2
Indexed BySCI
Language英语
WOS Research AreaIntegrative & Complementary Medicine ; Pharmacology & Pharmacy
WOS SubjectIntegrative & Complementary Medicine ; Pharmacology & Pharmacy
WOS IDWOS:000457136900001
Fulltext Access
Citation statistics
Cited Times [WOS]:3   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Avenida da Universidade, Taipa, Macao, China
2.Laboratory of Molecular Pharmacology, Department of Pharmacology, School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, China
3.Leiden University European Center for Chinese Medicine and Natural Compounds, Institute of Biology, Leiden University, Leiden, The Netherlands
4.School of Chinese Medicine, Hong Kong Baptist University, Kowloon, Hong Kong, China
First Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Yangyang Zhou,Hongxun Tao,Anqi Wang,et al. Chinese herb pair Paeoniae Radix Alba and Atractylodis Macrocephalae Rhizoma suppresses LPS-induced inflammatory response through inhibiting MAPK and NF-κB pathway[J]. CHINESE MEDICINE,2019,14.
APA Yangyang Zhou.,Hongxun Tao.,Anqi Wang.,Zhangfeng Zhong.,Xu Wu.,...&Yitao Wang.(2019).Chinese herb pair Paeoniae Radix Alba and Atractylodis Macrocephalae Rhizoma suppresses LPS-induced inflammatory response through inhibiting MAPK and NF-κB pathway.CHINESE MEDICINE,14.
MLA Yangyang Zhou,et al."Chinese herb pair Paeoniae Radix Alba and Atractylodis Macrocephalae Rhizoma suppresses LPS-induced inflammatory response through inhibiting MAPK and NF-κB pathway".CHINESE MEDICINE 14(2019).
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Yangyang Zhou]'s Articles
[Hongxun Tao]'s Articles
[Anqi Wang]'s Articles
Baidu academic
Similar articles in Baidu academic
[Yangyang Zhou]'s Articles
[Hongxun Tao]'s Articles
[Anqi Wang]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Yangyang Zhou]'s Articles
[Hongxun Tao]'s Articles
[Anqi Wang]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.