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Nanoencapsulation of Cyanidin-3-O-glucoside Enhances Protection Against UVB-Induced Epidermal Damage through Regulation of p53-Mediated Apoptosis in Mice
Liu, Zhaohan; Hu, Yunfeng; Li, Xia; Mei, Zhouxiong; Wu, Shi; He, Yong; Jiang, Xinwei; Sun, Jianxia; Xiao, Jianbo; Deng, Liehua; Bai, Weibin
2018-05-30
Conference NameJOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume66
Issue21
Pages5359-5367
Publication Place1155 16TH ST, NW, WASHINGTON, DC 20036 USA
PublisherAMER CHEMICAL SOC
AbstractExcess ultraviolet (UV) radiation causes numerous forms of skin damage. The aim of the present study was to assess and compare the photoprotective effects of cyanidin-3-O-glucoside (C3G) alone and encapsulated in chitosan nanoparticles (Nano-C3G) in a UVB-induced acute photodamage mouse model. Nano-C3G was developed from chitosan and sodium tripolyphosphate (TPP) by ionic gelation. The particle size, zeta potential, entrapment efficiency, drug loading, and in vitro release in 6 days were determined. Kunming (KM) mice were treated with Nano-C3G (125, 250, 500 mu M) or C3G (500 mu M) after part of the dorsal skin area was dehaired and then exposed to 2 J/cm(2) of UVB. The nanocapsules were successfully produced and had a uniform and complete spherical shape without agglomeration. The size, zeta potential, entrapment efficiency, and drug loading of Nano-C3G was 288 nm, +30 mV, 44.90%, and 4.30%, respectively. C3G in the nanocapsules was released quite rapidly, and the release rate slowed down at higher pH. The animal experiment demonstrated that Nano-C3G could effectively reduce the UVB-induced lipid peroxidation, malondialdehyde, and 8-hydroxy-2'-deoxyguanosine contents; downregulate p53, Bcl-2-associated X (Bax), and caspase-3 and -9 expression; and balance the B-cell lymphoma-2/leukemia-2 ratio. Moreover, Nano-C3G (125, 250, 500 MM) improved the visual appearance, skin moisture, histologic appearance, and apoptotic index (based on TUNEL staining) under UVB exposure. In conclusion, these results suggest that Nano-C3G can reduce UVB-induced epidermal damage through the p53-mediated apoptosis signaling pathway. Moreover, Nano-C3G was more efficient than C3G at the same concentration (500 mu M).
Keywordultraviolet B (UVB) cyanidin-3-O-glucoside (C3G) nanoparticles mitochondrial apoptosis skin photodamage
DOI10.1021/acs.jafc.8b01002
URLView the original
Indexed BySCI ; CPCI
Language英语
WOS Research AreaAgriculture ; Chemistry ; Food Science & Technology
WOS SubjectAgriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology
WOS IDWOS:000434100900013
The Source to ArticleWOS
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Cited Times [WOS]:2   [WOS Record]     [Related Records in WOS]
Document TypeConference paper
CollectionUniversity of Macau
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Liu, Zhaohan,Hu, Yunfeng,Li, Xia,et al. Nanoencapsulation of Cyanidin-3-O-glucoside Enhances Protection Against UVB-Induced Epidermal Damage through Regulation of p53-Mediated Apoptosis in Mice[C]. 1155 16TH ST, NW, WASHINGTON, DC 20036 USA:AMER CHEMICAL SOC,2018:5359-5367.
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